Novel molecularly imprinted polymer (MIP) multiple sensors for endogenous redox couples determination and their applications in lung cancer diagnosis
Novel molecularly imprinted polymer (MIP) multiple sensors for endogenous redox couples determination and their applications in lung cancer diagnosis
复制标题
新型分子印迹聚合物（MIP）多传感器用于内源性氧化还原对测定及其在肺癌诊断中的应用
DOI：
10.1016/j.talanta.2019.03.018
复制
发表时间：
2019
期刊：
影响因子：
6.1
通讯作者：
Li Yingchun
中科院分区：
文献类型：
--
作者：
Liu Jie;Wang Yuli;Liu Xiaoxue;Yuan Qunhui;Zhang Yang;Li Yingchun
开发了用于安培检测谷胱甘肽二硫化物（GSSG）、谷胱甘肽（GSH）、半胱氨酸（Cys）、胱氨酸（Cyss）、β-烟酰胺腺嘌呤二核苷酸磷酸（NADP+）和辅酶II还原型四钠盐（NADPH）的多重电化学传感器，其中对Cyss、NADP+和NADPH的分析是首次使用该传感系统进行分析。这些传感器的特异性由一层分子印迹聚合物（MIP）保证，该层以分析物为模板进行原位电聚合。所有传感器均使用标准缓冲液和小鼠血液样本进行测试，对相应分析物显示出令人满意的性能。六种分析物的动态浓度范围为 10−11–10−8mol/L，检测限低至 20 pmol/L。此外，电极上人工合成的MIP薄膜具有良好的选择性和稳定性。实际血样测量证明传感器具有良好的精度，回收率在92%~112%之间。更重要的是，使用所提出的传感器对肺癌患者和健康捐赠者的血液样本进行了分析。根据这些内源性物质的含量计算出氧化还原电位（Ehc），用来反映人体的健康状况，帮助诊断肺癌。肺癌患者GSH、NADPH和Ehc绝对值（GSH/GSSG）的水平显着低于健康人群（P<0.01），而GSSG的含量则较高（P<0.01）。血液检测结果提示GSH、NADPH、NADP+和Ehc(GSH/GSSG)含量可作为肺癌预诊断的生物标志物。与现有技术相比，这些用于癌症液体活检的新型传感器具有成本效益和可扩展性优势，有可能实现更广泛的临床访问和高效的人群筛查。
Multiplex electrochemical sensors for amperometric detection of glutathione disulfide (GSSG), glutathione (GSH), cysteine (Cys), cystine (Cyss),β-nicotinamide adenine dinucleotide phosphate (NADP+) and coenzyme II reduced tetrasodium salt (NADPH) were developed, in which analysis of Cyss, NADP+and NADPH are the first report using this sensing system. Specificity of these sensors were ensured by a layer of molecularly imprinted polymer (MIP) which was electropolymerized in situ with the analytes as template. All the sensors were tested with standard buffers and mouse blood samples, showing satisfactory performance towards the corresponding analytes. Dynamic concentration for the six analytes was in the range of 10−11–10−8mol/L with the detection limit down to 20 pmol/L. In addition, artificially synthesized MIP film on the electrodes allowed for good selectivity and stability. Real blood sample measurement proved that the sensors owned decent accuracy with recovery value ranging from 92%~112%. More importantly, blood samples from lung cancer patients and healthy donors were assayed by using the proposed sensors. Redox potentials (Ehc) were calculated based on the contents of these endogenic substances, which were utilized to reflect the health status of human body and help diagnose lung cancer. The levels of GSH, NADPH and the absolute value ofEhc(GSH/GSSG)in patients with lung cancer are significantly lower (P< 0.01) than those in healthy people, while the contents of GSSG (P< 0.01) are higher. The blood test results suggested that the content of GSH, NADPH, NADP+andEhc(GSH/GSSG)might serve as biomarkers for lung cancer prediagnosis. These novel sensors for liquid biospy of cancer have cost–benefit and scalability advantage over current techniques, potentially enabling broader clinical access and efficient population screening.