Isolation of human immunodeficiency virus from hemophiliacs: correlation with clinical symptoms and immunologic abnormalities.
Isolation of human immunodeficiency virus from hemophiliacs: correlation with clinical symptoms and immunologic abnormalities.
复制标题
从血友病患者中分离人类免疫缺陷病毒：与临床症状和免疫异常的相关性。
DOI：
10.1016/s0022-3476(87)80241-x
复制
发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Levine,PH
中科院分区：
文献类型：
--
作者：
Andrews,CA;Sullivan,JL;Brettler,DB;Brewster,FE;Forsberg,AD;Scesney,S;Levine,PH
作为血友病患者人类免疫缺陷病毒（HIV）感染的前瞻性研究的一部分，对72名无获得性免疫缺陷综合征（AIDS）或AIDS相关综合征（ARC）的个体的外周血单核细胞（PBM）进行病毒培养。HIV分离PBMs从16（24%）的66例血友病谁是HIV血清阳性，没有6个血清阴性患者。从6名患者中分离出HIV的5名患者的细胞在3至12个月后再次成功培养出病毒。在15例HIV阳性细胞患者中的7例和50例HIV阴性细胞患者中的8例的血清中检测到HIV核心P24抗原。分离阳性细胞的血友病患者的辅助细胞数量显著少于那些细胞在培养中不产生HIV的患者，辅助/T抑制细胞比例、美洲商陆有丝分裂原反应性和血小板总数显著低于那些细胞在培养中不产生HIV的患者。HIV中和抗体滴度在有或没有HIV阳性PBMs的血友病患者之间没有差异。在研究的18个月内，16名病毒阳性细胞的患者中有3人发展为艾滋病，2人发展为ARC，而50名血清阳性血友病患者中有3人的细胞没有产生病毒，他们在同一时期发展为ARC。辅助性T细胞数量的显著减少，血小板计数的减少，以及HIV分离组中进展为AIDS的更高速率可能反映了更重的病毒载量，表明培养HIV的能力可能是更重要疾病的早期标志。
As part of a prospective study of human immunodeficiency virus (HIV) infection in hemophilia, peripheral blood mononuclear cells (PBMs) from 72 individuals without acquired immune deficiency syndrome (AIDS) or AIDS-related complex (ARC) were cultured for virus. HIV was isolated from PBMs from 16 (24%) of 66 patients with hemophilia who were seropositive for HIV and from none of six seronegative patients. Cells from five of six patients from which HIV was isolated were again successfully cultured for virus 3 to 12 months later. HIV core P24 antigen was detected in serum from seven of 15 patients with HIV-positive cells and from eight of 50 with HIV-negative cells. Patients with hemophilia with isolation-positive cells had significantly fewer ^ helper cells and significantly lower ^ helper/T suppressor ratios, pokeweed mitogen responsiveness, and total platelet counts than did those whose cells did not yield HIV on cultivation. HIV neutralizing antibody titers did not differ between hemophiliacs with or without HIV-positive PBMs. Three of the 16 patients with virus-positive cells developed AIDS, and two ARC, within 18 months of the study, compared with three of 50 seropositive hemophiliacs whose cells did not yield virus, who developed ARC during the same period. The significant decrease in the number of T helper cells, decreased platelet counts, and higher rate of progression to AIDS in the group with HIV isolation may reflect a heavier virus load, indicating that the ability to culture HIV may be an early marker of more significant disease.