Simple diagnostic assay for hairy cell leukaemia by immunocytochemical detection of annexin A1 (ANXA1)
Simple diagnostic assay for hairy cell leukaemia by immunocytochemical detection of annexin A1 (ANXA1)
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DOI：
10.1016/s0140-6736(04)16356-3
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发表时间：
2004-06-05
期刊：
影响因子：
168.9
通讯作者：
Pileri, S
中科院分区：
文献类型：
--
作者：
Falini, B;Tiacci, E;Pileri, S
能够准确地区分毛细胞白血病和其他 B 细胞恶性疾病的标记物将具有巨大的诊断价值。通过基因表达谱分析，膜联蛋白 A1 (ANXA1) 已被确定为在毛细胞白血病中表达上调的基因。我们使用特异性抗 ANXA1 单克隆抗体对 500 个 B 细胞肿瘤进行了免疫染色，结果表明 ANXA1 蛋白表达对毛细胞白血病具有特异性。 ANXA1 的免疫细胞化学检测代表了一种简单、廉价、高灵敏度和特异性 (100%) 的毛细胞白血病诊断检测方法。该测定对于区分毛细胞白血病与绒毛淋巴细胞脾淋巴瘤和变异毛细胞白血病特别有用，这两种疾病通常对毛细胞白血病有效的治疗反应不佳。
A marker capable of distinguishing with certainty between hairy cell leukaemia and other B-cell malignant disease would be of great diagnostic value. Through gene expression profiling, annexin A1 (ANXA1) has been identified as a gene that is upregulated in hairy cell leukaemia. We did immunostaining of 500 B-cell tumours with a specific anti-ANXA1 monoclonal antibody and showed that ANXA1 protein expression is specific to hairy cell leukaemia. Immunocytochemical detection of ANXA1 represents a simple, inexpensive, highly sensitive and specific (100%) assay for diagnosis of hairy cell leukaemia. This assay will be especially useful in distinguishing hairy cell leukaemia from splenic lymphoma with villous lymphocytes and variant hairy cell leukaemia, both of which usually respond poorly to treatments that are effective in hairy cell leukaemia.