CHARACTERIZATION OF APOLIPOPROTEINS FROM CHICKEN PLASMA
CHARACTERIZATION OF APOLIPOPROTEINS FROM CHICKEN PLASMA
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DOI：
10.1016/s0021-9673(01)89486-3
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发表时间：
1990-07-20
期刊：
影响因子：
--
通讯作者：
DOWER, HJ
中科院分区：
文献类型：
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作者：
BROWN, EM;DOWER, HJ
虽然功能相似，但鸟类和哺乳动物的脂蛋白系统在组成上有所不同。主要的载脂蛋白，载脂蛋白A-I和载脂蛋白B，在所有脊椎动物中都是常见的；然而，载脂蛋白A-II和载脂蛋白E是哺乳动物脂蛋白的重要功能成分，在鸡血浆中不存在。鸡载脂蛋白A-I和载脂蛋白B已经被表征，并且在鸡脂蛋白的电泳模式中观察到几种次要的载脂蛋白成分。在本研究中，采用单密度梯度超离心分离鸡脂蛋白并将其分解成不同的密度等级。分离的脂蛋白用己烷-丙醇（3:2）降解。载脂蛋白在pH 8.5下溶于3Mguanidine hydrochloride中，在25 × 0.4 cm c4反相柱上用0.1%三氟乙酸在乙腈梯度中进行色谱。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定的载脂蛋白分子量和氨基酸组成与其他物种的载脂蛋白进行了比较，以寻找功能上的相似性。观察到鸡的主要载脂蛋白与人的几种载脂蛋白在组成上的相似性。
Although functionally similar, the lipoprotein systems of birds and mammals differ in composition. the major apolipoproteins, apo A-I and apo B, are common to all vertebrates; however apo A-II and apo E, functionally important components of mammalian lipoproteins, are absent from chicken plasma. Chicken apo A-I and apo B have been characterized, and several minor apolipoprotein components have been observed in electrophoretic patterns of chicken lipoproteins. In this study a single density gradient ultracentrifugation was used to isolate and subfractionate chicken lipoproteins into density classes. Isolated lipoproteins were delipidated with hexane-sipopropanol (3:2). Apolipoproteins were then solubilized at pH 8.5 in 3Mguanidine hydrochloride and chromatographed on a 25 × 0.4 cm C4reversed-phase column using 0.1% trifluoroacetic acid in a gradient of acetonitrile in water. Molecular weights estimated by sodium dodecyl sulfate—polyacrylamide gel electrophoresis and amino acid compositions were compared with those of apolipoproteins from other species in a search for functional similarities. Similarities in composition between the major chicken apolipoprotein and several human apolipoproteins were observed.