Identification of a novel protective human monoclonal antibody, LXY8, that targets the key neutralizing epitopes of staphylococcal enterotoxin B
Identification of a novel protective human monoclonal antibody, LXY8, that targets the key neutralizing epitopes of staphylococcal enterotoxin B
复制标题
鉴定一种新型保护性人单克隆抗体 LXY8，该抗体针对葡萄球菌肠毒素 B 的关键中和表位
DOI：
10.1016/j.bbrc.2021.02.057
复制
发表时间：
2021-03-02
影响因子：
3.1
通讯作者：
Luo, Longlong
中科院分区：
文献类型：
--
作者：
Hu, Naijing;Qiao, Chunxia;Luo, Longlong
Staphylococcal enterotoxin B (SEB), one of the exotoxins produced by Staphylococcus aureus, is the key toxin that causes poisoning reactions and toxic shock syndrome. In the current research work, a novel human antibody named LXY8 was screened from a human phage display antibody library, and LXY8 blocked the interaction between SEB and the T cell receptor (TCR). The binding activity between LXY8 and SEB was 0.525 nM. Furthermore, LXY8 could effectively inhibit the SEB-induced activation of peripheral blood mononuclear cells and release of cytokines. In the BALB/c mouse model, LXY8 effectively neutralized SEB toxicity in vivo. Finally, based on computer-guided molecular modeling, we designed a series of SEB mutation sites; these sites facilitated the determination of the key residues (i.e. (EFNN179)-E-176) of SEB recognized by LXY8. The research revealed that the (EFNN179)-E-176 residues of SEB are important for specific antibody-antigen recognition. The results may be helpful for the development of antibody-based therapy for SEB-induced toxic shock syndrome. (C) 2021 Elsevier Inc. All rights reserved.
