Phosphotyrosine-modified proteins are concentrated at the membranes of epithelial and endothelial cells during tissue development in chick embryos.
Phosphotyrosine-modified proteins are concentrated at the membranes of epithelial and endothelial cells during tissue development in chick embryos.
复制标题
在雏鸡胚胎的组织发育过程中，磷酸酪氨酸改性的蛋白浓缩在上皮细胞和内皮细胞的膜上。
DOI：
10.1083/jcb.106.5.1757
复制
发表时间：
1988-05
影响因子：
7.8
通讯作者：
Singer, S J
中科院分区：
文献类型：
--
作者：
Takata, K;Singer, S J
我们已经使用了高亲和力的多克隆抗体特异性磷酸酪氨酸（PTYR）残基，检查本地化在各种鸡胚组织原位的PTYR修饰蛋白的免疫细胞化学方法。在发育的9 - 21天期间，大多数组织表现出升高水平的PTyr修饰的蛋白，如通过用抗PTyr抗体对组织提取物进行免疫印迹实验所测定的（Maher，P.A.，和e. B。帕斯夸里1988. 106：1747 - 1755）。通过半薄冷冻切片的免疫荧光标记，在所有检查的胚胎组织中最高浓度的PTYR免疫标记定位于上皮细胞和内皮细胞的膜，其他细胞未显示出可检测的标记。这些结果证实了免疫电子显微镜标记，这表明特别高浓度的PTYR修饰的蛋白质接近膜的顶端交界处。相应的成体组织未显示标记。建议这些结果反映了胚胎发育过程中上皮和内皮细胞连接的功能可塑性的分子基础。
We have used high affinity polyclonal antibodies specific for phosphotyrosine (PTyr) residues to examine the localization in various chick embryonic tissues in situ of PTyr-modified proteins by immunocytochemical methods. During the period from 9 to 21 d of development, most tissues exhibit elevated levels of PTyr-modified proteins as determined by immunoblotting experiments of tissue extracts with the anti-PTyr antibodies (Maher, P. A., and E. B. Pasquale. 1988. J. Cell Biol. 106:1747-1755). By immunofluorescence labeling of semithin frozen sections, the highest concentrations of PTyr immunolabeling in all of the embryonic tissues examined were localized to the membranes of the epithelial and endothelial cells with other cells showing no detectable labeling. These results were confirmed by immunoelectron microscopic labeling, which showed particularly high concentrations of PTyr-modified proteins close to the membranes at the apical junctions. The corresponding adult tissues showed no labeling. It is proposed that these results reflect the molecular basis for the functional plasticity of epithelial and endothelial cell junctions during embryonic development.