Core-shell iron oxide-layered double hydroxide: High electrochemical sensing performance of H2O2 biomarker in live cancer cells with plasma therapeutics
Core-shell iron oxide-layered double hydroxide: High electrochemical sensing performance of H2O2 biomarker in live cancer cells with plasma therapeutics
复制标题
核壳氧化铁层状双氢氧化物：采用等离子体疗法对活癌细胞中的 H2O2 生物标志物进行高电化学传感性能
DOI：
10.1016/j.bios.2017.05.057
复制
发表时间：
2017-11-15
影响因子：
12.6
通讯作者：
Liu, Hongfang
中科院分区：
文献类型：
--
作者：
Asif, Muhammad;Liu, Hongwei;Liu, Hongfang
本论文通过在铁氧化物（Fe 3 O 4）纳米球（NS）表面可控地整合CuAl层状双金属氢氧化物（LDHs），制备了一种新型的多功能核壳纳米材料（Fe3O4@CuAl NS），并探索了其在新兴生物标志物（即，H2 O2作为癌症诊断探针。此外，大气压等离子体（APPs）也已被用作癌症治疗的潜在治疗方法。由于水滑石的p型孔道与多功能特性、独特的形貌和丰富的表面活性中心的协同作用，Fe3O4@CuAlNSs修饰电极对H2 O2的还原具有良好的电催化活性。在优化条件下，该传感器对H2 O2具有良好的电化学传感性能，线性范围宽达8个数量级，真实的检测限为1 nM（S/N = 3），灵敏度高，重现性好，长期稳定性好。由于电化学生物传感器的高效率，已被用于在体外测定人尿液和血清样品中的H2 O2浓度之前和之后的摄入咖啡，并实时监测H2 O2流出从不同的癌细胞系在正常状态下和血浆治疗后。我们相信，这种新型的结构集成的核-壳纳米杂化材料与APP相结合的纳米平台将提高癌症疾病的诊断和治疗窗口。
In this work, we develop a new type of multifunctional core-shell nanomaterial by controllable integration of CuAl layered double hydroxides (LDHs) over the surface of iron oxides (Fe3O4) nanospheres (NSs) to fabricate (Fe3O4@CuAl NSs) hybrid material with interior tunability of LDH phase and explore its practical application in ultrasensitive detection of emerging biomarker, i.e., H2O2 as cancer diagnostic probe. In addition, atmospheric pressure plasmas (APPs) have also been used as potential therapeutic approach for cancer treatment. Due to the synergistic combination of p-type semiconductive channels of LDHs with multi-functional properties, unique morphology and abundant surface active sites, the Fe3O4@CuAl NSs modified electrode exhibited attractive electrocatalytic activity towards H2O2 reduction. Under the optimized conditions, the proposed biosensor demonstrated striking electrochemical sensing performances to H2O2 including linear range as broad as 8 orders of magnitude, low real detection limit of 1 nM (S/N = 3), high sensitivity, good reproducibility and longterm stability. Arising from the superb efficiency, the electrochemical biosensor has been used for in vitro determination of H2O2 concentrations in human urine and serum samples prior to and following the intake of coffee, and real-time monitoring of H2O2 efflux from different cancer cell lines in normal state and after plasma treatment. We believe that this novel nano-platform of structurally integrated core-shell nanohybrid materials combined with APPs will enhance diagnostic as well as therapeutic window for cancer diseases.