Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia, Oreochromis niloticus
Micrococcus luteus and Pseudomonas species as probiotics for promoting the growth performance and health of Nile tilapia, Oreochromis niloticus
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DOI：
10.1016/j.fsi.2009.03.020
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发表时间：
2009-08-01
影响因子：
4.7
通讯作者：
Shalaby, Adel M. E.
中科院分区：
文献类型：
--
作者：
Abd El-Rhman, Azza M.;Khattab, Yassir A. E.;Shalaby, Adel M. E.
从尼罗罗非鱼 Oreochromis niloticus 的性腺和肠道中分离出藤黄微球菌和假单胞菌属。 M. uteus 和 Ps。种拮抗嗜水气单胞菌，抑菌圈直径分别为 4 和 9 厘米。将这两种微生物添加到含有 30% 粗蛋白的人工基础饲料中，除了一些血液参数和化学参数外，还评估它们对生长性能和存活率的功效。将 240 条平均体重为 2.35 +/- 0.1 g/鱼的尼罗罗非鱼平均分为 4 个处理，每组重复。 T-1给予基础饮食（对照），T-2给予含有藤黄杆菌的基础饮食，T-3给予含有Ps的基础饮食。种，T-4 的基础饮食中含有 M. uteus 和 Ps. 。物种。在实验期间（90天），每周喂鱼六天，每天喂鱼两次，喂食量为活体重的3%。通过腹腔注射用嗜水气单胞菌(0.3 x 10(7) cells ml(-1))攻击鱼，并继续饲养14天。T-2的生长率、饲料利用率和存活率均最佳。 T-2 的红细胞计数显着高于 T-3 和对照 T-1。 T-3 和 T-4 的血细胞比容值 (HCT) 和血红蛋白含量 (Hb) 显着降低。 T-3 记录了最高的葡萄糖水平。 T-2的受攻击鱼的死亡率为25%，而T-1、T-3和T-4的受攻击鱼的死亡率为80-90%。藤黄素增强了鱼类的生长和健康。建议使用藤黄微球菌作为体内益生菌，而假单胞菌仅在体外表现出益生菌作用。 (C) 2009 Elsevier Ltd. 保留所有权利。
Micrococcus luteus and Pseudomonas species were isolated from the gonads and intestine of Nile tilapia, Oreochromis niloticus. M. luteus and Ps. species antagonized Aeromonas hydrophila with inhibition zone of 4 and 9 cm diameter, respectively. Both microorganisms were added to artificial basal diet with 30% crude protein to evaluate their efficacy on the growth-performance and survival rate, besides some blood-parameters and chemistry. Two hundred and forty O. niloticus with average body-weight of 2.35 +/- 0.1 g/fish were equally divided into four treatments of triplet replicates. T-1 was given basal diet (control), T-2 was given basal diet with M. luteus, T-3 was given basal diet with Ps. species, T-4 was given basal diet with both M. luteus and Ps. Species. Fishes were fed twice daily at the rate of 3% of their live body-weight, for six days a week during the experimental periods (90 day). Fish were challenged by A. hydrophila (0.3 x 10(7) cells ml(-1)) via intra-peritoneal injection and kept for 14 more days.The best growth rate, feed utilization and survival rate were noticed with T-2. The erythrocyte-count was significantly higher with T-2 than T-3 than the control T-1. The hematocrit-values (HCT) and hemoglobin-content (Hb) were significantly decreased with T-3 and T-4. The highest glucose-level was recorded with T-3. The challenged fish of T-2 showed 25% mortality, while T-1, T-3 and T-4 showed 80-90% mortality.M. luteus enhanced the fish growth and health. It is recommended to use M. luteus as a probiotic in vivo, while Pseudomonas species showed probiotic effects in vitro only. (C) 2009 Elsevier Ltd. All rights reserved.