A STUDY OF THERMOPHILIC CAMPYLOBACTERS IN A RIVER SYSTEM
A STUDY OF THERMOPHILIC CAMPYLOBACTERS IN A RIVER SYSTEM
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DOI：
10.1111/j.1365-2672.1987.tb02395.x
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发表时间：
1987-02-01
期刊：
影响因子：
--
通讯作者：
GODFREE, AF
中科院分区：
文献类型：
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作者：
BOLTON, FJ;COATES, D;GODFREE, AF
在12个半月的时间里，研究了一条流经农村和城市地区的15公里长的河流系统，该系统受到污水处理厂的排放。采用玻璃微纤维过滤法和最可能数（MPN）法，从12个地点每隔14 d采集312份样品。过滤法和MPN法分别检出43%和21%的弯曲杆菌。检出频率最低，弯曲杆菌数量最少（< 10个/100 ml）的样本来自农村和河流湍急的河段。弯曲杆菌的分离频率和数量最高（10-230个/100毫升）与污水处理厂附近或下游的地点有关。有明显的季节性趋势；秋末和冬季是分离株最多、数量最多的季节，春夏是分离株最少、数量最少的季节。暴雨后邻近农田的地表水径流也增加了河流系统中弯曲杆菌的数量。分离物的生物分型表明，最常见的弯曲杆菌属是空肠弯曲杆菌，但也分离到了大肠杆菌、laridis弯曲杆菌和以前未被识别的弯曲杆菌群。血清分型分化出14种空肠梭菌、11种大肠梭菌和2种laridis梭菌血清型。此外，在本研究中检测的河水中也经常发现从人肠炎中分离出的空肠梭菌血清型。
Fifteen kilometers of a river system traversing rural and urban areas and subject to sewage works effluent discharge was studied during a 12 1/2 month period. A total of 312 samples was collected from 12 sites at 14 d intervals and tested by a glass microfibre filtration method and a most probable number (MPN) method. Campylobacters were found in 43% of samples by the filtration method and 21% by the MPN method. The lowest frequency of isolation and lowest counts (< 10 campylobacters/100 ml) were associated with samples collected from rural sites and fast-flowing stretches of river. The greastest frequency of isolation and highest counts (> 10-230 campylobacters/100 ml) were associated with sites adjacent to or downstream of sewage works. There was an obvious seasonal trend; most isolations and highest counts were obtained in late autumn and winter, and fewest isolations and lowest counts in spring and summer. Surface water run-off from adjacent farmland following heavy rainfall also increased the counts of campylobacters in the river system. Biotyping of isolates demonstrated that the most prevalent Campylobacter sp. was Campylobacter jejuni but C. coli, C. laridis and a previously unrecognized group of campylobacters were also isolated. Serotyping differentiated 14 serotypes of C. jejuni, 11 of C. coli and two of C. laridis. Furthermore, serotypes of C. jejuni commonly isolated from enteritis in man were frequently found in river water tested during this study.