Characterization of a cDNA coding for sex steroid-binding protein of human plasma.
Characterization of a cDNA coding for sex steroid-binding protein of human plasma.
复制标题
编码人血浆性类固醇结合蛋白的 cDNA 的表征。
DOI：
10.1016/0014-5793(87)80261-2
复制
发表时间：
1987
期刊：
影响因子：
3.5
通讯作者：
Petra,PH
中科院分区：
文献类型：
--
作者：
Que,BG;Petra,PH
从先前通过用大鼠雄激素结合蛋白（ABP）cDNA筛选Charon 21A人肝cDNA文库分离的噬菌体克隆中鉴定了编码人血浆性类固醇结合蛋白（SBP）的cDNA（912个核苷酸）。从cDNA推导的氨基酸序列表明，插入片段是编码281个氨基酸的部分克隆，从残基92（甘氨酸）开始，包括交替的亮氨酰残基和羧基末端373（组氨酸），如先前报道的[（1986）Biochemistry 25，7584]。潜在的多聚腺苷酸化信号序列ATTAAA作为3′编码区和终止密码子TAA的一部分存在。两个序列之后都是短的20个未翻译核苷酸和49个核苷酸的poly（A）序列。人SBP和大鼠ABP在DNA水平上存在显著的同源性（76%），这可能表明两者都是从一个共同的原始基因进化而来的。在人肝cDNA文库中存在SBP cDNA的证明提供了肝脏是SBP生物合成位点的第一个证据。
A cDNA (912 nucleotides) coding for human plasma sex steroid‐binding protein (SBP) was characterized from a phage clone previously isolated by screening a Charon 21A human liver cDNA library with rat androgen binding protein (ABP) cDNA. The deduced amino acid sequence from the cDNA indicated that the insert was a partial clone coding for 281 amino acids starting with residue 92 (glycine) encompassing the alternating leucyl residues and the carboxyl‐end 373 (histidine) as previously reported [(1986) Biochemistry 25, 7584]. The potential polyadenylation signal sequence ATTAAA is present as part of the 3′‐coding region and the stop codon TAA. Both are followed by a short 20 untranslated nucleotides and a poly(A) tract of 49 nucleotides. Significant homologous sequences (76%) at the DNA level exist between human SBP and rat ABP which might suggest the possibility that both evolved from a common primordial gene. Demonstration of the presence of an SBP cDNA in a human liver cDNA library provides the first evidence that liver is the site of SBP biosynthesis.