Poorly differentiated desmin-negative and vimentin-positive leiomyosarcoma of the stomach examined by the immunohistochemical and quick-freezing and deep-etching methods
Poorly differentiated desmin-negative and vimentin-positive leiomyosarcoma of the stomach examined by the immunohistochemical and quick-freezing and deep-etching methods
复制标题
免疫组化及速冻深蚀法检测低分化结蛋白阴性和波形蛋白阳性胃平滑肌肉瘤
DOI：
10.1007/s004280050181
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
A. Kawaoi
中科院分区：
文献类型：
--
作者：
A. Hemmi;A. Komiyama;S. Ohno;Y. Fujii;R. Katoh,;A. Yokoyama;A. Kawaoi
摘要：报道了一名 41 岁女性的胃低分化平滑肌肉瘤。通过对 HHF35 的弥漫性免疫组织化学反应以及通过常规电子显微镜在一些肿瘤细胞中出现局灶密度和凹坑证实了诊断。免疫组织化学显示，大多数肿瘤细胞具有未分化的性质，结蛋白、α-平滑肌肌动蛋白和IV型胶原免疫染色呈阴性，波形蛋白免疫染色呈阳性。通过快速冷冻和深蚀刻（QF-DE）方法，这些肿瘤细胞显示出成束的肌动蛋白和肌球蛋白丝的丢失，这些肌动蛋白丝和肌球蛋白丝构成了结蛋白相关结构（局灶密度和致密斑片区域）。它们的细胞质有许多线粒体和其他细胞器。在细胞器间空间中观察到中间丝（IF），通过免疫组织化学确定为波形蛋白，并与这些细胞器相连。肌动蛋白丝形成网状结构，主要分布在质膜下区域。尽管传统电子显微镜未检测到基底层，但观察到基底层样结构，即细胞外基质和细胞膜之间的关联。采用QF-DE方法，观察平滑肌肉瘤细胞骨架和细胞外基质的三维超微结构改变。
Abstract A poorly differentiated leiomyosarcoma of the stomach in a 41-year-old woman is reported. The diagnosis was confirmed by the diffuse immunohistochemical reaction to HHF35, and the presence of focal density and caveolas in some of the tumour cells by conventional electron microscopy. Immunohistochemically, most tumour cells had an undifferentiated nature, in which negative immunostaining for desmin, alpha-smooth muscle actin, and type IV collagen, and positive immunostaining for vimentin were observed. By the quick-freezing and deep-etching (QF-DE) method, these tumour cells revealed the loss of bundled actin and myosin filaments, which constitute desmin associated structures (focal densities and dense patchy areas). Their cytoplasm had many mitochondria and other cell organelles. The intermediate filaments (IFs), which were determined to be vimentin by immunohistochemistry, were observed in the inter-organellar spaces, and connected with these cell organelles. Actin filaments formed a meshwork structure and were distributed mainly in subplasmalemmal regions. Although a basal lamina was not detected by conventional electron microscopy, basal lamina-like structures, an association between the extracellular matrices and the cell membrane, were observed. Using the QF-DE method, three dimensional ultrastructural alterations of the cytoskeleton and extracellular matrix of the leiomyosarcoma were observed.