Chronically implanted Neuropixels probes enable high-yield recordings in freely moving mice
Chronically implanted Neuropixels probes enable high-yield recordings in freely moving mice
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DOI：
10.7554/elife.47188
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发表时间：
2019-08-14
期刊：
影响因子：
7.7
通讯作者：
Churchland, Anne K.
中科院分区：
文献类型：
--
作者：
Juavinett, Ashley L.;Bekheet, George;Churchland, Anne K.
使用Neuropixels探针的高产量电生理学的出现现在使研究人员能够同时记录数百个具有显着高信噪比的神经元。然而，这些探针并不适合用于自由移动的小鼠。由于许多原因，研究不受限制的动物的神经活动至关重要，例如利用行为学方法研究神经回路。我们设计并实现了一种新的设备，允许Neuropixels探针被定制用于在自由移动的小鼠中进行长期植入实验。我们证明了这种方法的易用性和实用性，在数周的实验中记录数百个神经元的行为学行为。我们为其他研究人员提供技术图纸和程序。重要的是，我们的方法使研究人员能够取出并重复使用这些有价值的探针，这是一个变革性的步骤，尚未建立任何类型的长期植入探针的记录。
The advent of high-yield electrophysiology using Neuropixels probes is now enabling researchers to simultaneously record hundreds of neurons with remarkably high signal to noise. However, these probes have not been well-suited to use in freely moving mice. It is critical to study neural activity in unrestricted animals for many reasons, such as leveraging ethological approaches to study neural circuits. We designed and implemented a novel device that allows Neuropixels probes to be customized for chronically implanted experiments in freely moving mice. We demonstrate the ease and utility of this approach in recording hundreds of neurons during an ethological behavior across weeks of experiments. We provide the technical drawings and procedures for other researchers to do the same. Importantly, our approach enables researchers to explant and reuse these valuable probes, a transformative step which has not been established for recordings with any type of chronically-implanted probe.