Histone acetylation regulates the time of replication origin firing
Histone acetylation regulates the time of replication origin firing
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DOI：
10.1016/s1097-2765(02)00702-5
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发表时间：
2002-11-01
期刊：
影响因子：
16
通讯作者：
Grunstein, M
中科院分区：
文献类型：
--
作者：
Vogelauer, M;Rubbi, L;Grunstein, M
酵母中整个 S 期复制起点的时间激发取决于相邻染色体环境中的未知决定因素。我们在此证明，周围染色质的组蛋白乙酰化状态是颞发射的重要调节因子。 RPD3 组蛋白脱乙酰酶的缺失会导致较早的起点激发，并同时使复制因子 Cdc45p 与起点结合。此外，通过组蛋白乙酰转移酶 Gcn5p 的募集，增加了晚期起源 ARS1412 附近历史的乙酰化，导致单独的 ARS1412 更早启动。这些数据表明组蛋白乙酰化是起始放电时间的直接决定因素。
The temporal firing of replication origins throughout S phase in yeast depends on unknown determinants within the adjacent chromosomal environment. We demonstrate here that the state of histone acetylation of surrounding chromatin is an important regulator of temporal firing. Deletion of RPD3 histone deacetylase causes earlier origin firing and concurrent binding of the replication factor Cdc45p to origins. In addition increased acetylation of histories in the vicinity of the late origin ARS1412 by recruitment of the histone acetyltransferase Gcn5p causes ARS1412 alone to fire earlier. These data indicate that histone acetylation is a direct determinant of the timing of origin firing.