Regulation of DNA nucleases by molecular crowding.
Regulation of DNA nucleases by molecular crowding.
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DOI：
10.1093/nar/gkm445
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发表时间：
2007
影响因子：
14.9
通讯作者：
Sugimoto N
中科院分区：
文献类型：
--
作者：
Sasaki Y;Miyoshi D;Sugimoto N
在这里，我们研究了分子拥挤对核酸酶的功能，结构和稳定性的影响。我们发现，一个29-mer的双链DNA的核酸内切酶DNase I和S1核酸酶的水解大大提高了分子拥挤使用聚乙二醇（PEG），然而，分子拥挤对水解的影响不大外切III和外切I核酸外切酶。此外，动力学分析表明，20%（w/v）PEG分子拥挤使DNase I在25°C的最大反应速度从0.1 μM/min增加到2.7 μM/min，而核酸外切酶I在37°C的最大反应速度从2.2 μM/min降低到0.4 μM/min。这些结果表明，分子拥挤对核酸外切酶和核酸内切酶的催化活性有不同的影响。
Here, we examined the effects of molecular crowding on the function, structure and stability of nucleases. We found that the hydrolysis of a 29-mer double-stranded DNA by the endonucleases DNase I and S1 nuclease was substantially enhanced by molecular crowding using polyethylene glycol (PEG); however, molecular crowding had little effect on hydrolysis by exo III and exo I exonucleases. Moreover, kinetic analysis showed that the maximum velocity for the reaction of DNase I at 25°C was increased from 0.1 to 2.7 μM/min by molecular crowding with 20% (w/v) PEG, whereas that of exonuclease I at 37°C decreased from 2.2 to 0.4 μM/min. In contrast, molecular crowding did not significantly affect the Michaelis constant of DNase I or exonuclease I. These results indicate that molecular crowding has different effects on the catalytic activities of exonucleases and endonucleases.
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影响因子：
30.8
作者：
Napirei, M;Karsunky, H;Möröy, T
通讯作者：
Möröy, T
影响因子：
14.9
作者：
KLUG, A;LUTTER, LC
通讯作者：
LUTTER, LC
影响因子：
14.9
作者：
NOLL, M
通讯作者：
NOLL, M
影响因子：
--
作者：
Napirei, M;Wulf, S;Mannherz, HG
通讯作者：
Mannherz, HG
影响因子：
4.8
作者：
Hatters, DM;Minton, AP;Howlett, GJ
通讯作者：
Howlett, GJ