Effects of ceftriaxone‐induced intestinal dysbacteriosis on regulatory T cells validated by anaphylactic mice
Effects of ceftriaxone‐induced intestinal dysbacteriosis on regulatory T cells validated by anaphylactic mice
复制标题
DOI：
10.1016/j.intimp.2018.05.003
复制
发表时间：
2018-05
影响因子：
5.6
通讯作者：
Xia Luo;Zeng-feng Pan;S. Luo;Qi Liu;Shao-wei Huang;Guanghua Yang;Feifei Nong;Ya-jun Fu;Xiangliang Deng;Lian Zhou
中科院分区：
文献类型：
--
作者：
Xia Luo;Zeng-feng Pan;S. Luo;Qi Liu;Shao-wei Huang;Guanghua Yang;Feifei Nong;Ya-jun Fu;Xiangliang Deng;Lian Zhou
人体肠道中的益生菌和病原体都表达病原体相关分子模式（PAMPs），并随着内毒素和细菌DNA的释放而死亡，这可以刺激我们的免疫系统并引起免疫反应。然而，解释为什么正常的肠道植物群不会像病原体那样产生免疫排斥反应是有趣和迷人的。本研究通过观察头孢曲松菌群失调小鼠肠道免疫耐受相关细胞和分子的变化，发现菌群失调小鼠肠道植物群的均匀度指数和Shannon Wiener指数均降低。此外，派伊尔集合淋巴结（PP）、肠系膜淋巴结（MLN）和脾脏（SP）中αβ+TCR+ CD 3 + CD 4 − CD 8 −细胞、CD 3 +γδTCR+细胞和CD 4 + CD 25 + FoxP 3+细胞的比例以及血清中TGF-β1、IL-2、IL-4和IL-10的水平也发生了变化。在哮喘小鼠模型中，肠道菌群失调导致对过敏原卵清蛋白（OVA）的免疫应答增强，卵清蛋白是一种加重哮喘症状的物质。总之，维持一定数量或种类的肠道微生物群落对于调节性T细胞在避免超敏反应中起作用是不可或缺的。
Both probiotics and pathogens in the human gut express pathogen-associated molecular patterns (PAMPs) and die with the release of endotoxin and bacterial DNA, which can stimulate our immune system and cause immune reaction. However, it's interesting and fascinating to address why the normal intestinal flora will not generate immunological rejection like the pathogen does. By investigating the changes in cells and molecules relevant to immune tolerance in mice with ceftriaxone-induced dysbacteriosis, our study discovered that both the Evenness indexes and Shannon Wiener index of intestinal flora showed a decrease in dysbacteriosis mice. Moreover, the proportion of αβ+TCR+CD3+CD4−CD8−cells, CD3+γδTCR+cells and CD4+CD25+FoxP3+cells in the Peyer's patches (PPs), mesenteric lymph nodes (MLNs) and spleen (SP) and the level of TGF-β1, IL-2, IL-4 and IL-10 in the serum also changed. Intestinal dysbacteriosis in an asthma murine model resulted in enhancement of immunologic response to the allergen ovalbumin (OVA), which was an agent that aggravates asthma symptoms. In summary, it is integral to maintain a certain amount or variety of intestinal microflora for regulatory T cells to act in averting hypersensitivity.