miR-29b Expression Is Associated With Disease-Free Survival in Patients With Ovarian Serous Carcinoma
miR-29b Expression Is Associated With Disease-Free Survival in Patients With Ovarian Serous Carcinoma
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DOI：
10.1111/igc.0b013e3181a48cf9
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发表时间：
2009-05-01
影响因子：
4.8
通讯作者：
O'Leary, John J.
中科院分区：
文献类型：
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作者：
Flavin, Richard;Smyth, Paul;O'Leary, John J.
Micro-RNAs是一组长度约为22个核苷酸的非编码小RNA。最近的研究表明，成熟的微小RNA在人类癌症中的差异表达。我们研究了miR-29b在卵巢浆液性癌中的表达变化。应用定量干环逆转录聚合酶链式反应分析50例常规固定、石蜡包埋的卵巢浆液性癌组织中miR-29b的表达。在相应的组织芯片中定量检测P53、雌激素受体、孕激素受体、人表皮生长因子受体2、Ki-67和胰岛素样生长因子I的蛋白表达。MiR-29b的表达与临床病理和患者生存相关。我们提供了确凿的证据，表明miR-29b在相当大比例的卵巢浆液性癌中表达下调，并与特定的临床病理特征有关，最显著的是miR-29b的高表达与无病生存率降低有关。
Micro-RNAs are a group of small noncoding RNAs approximately 22 nucleotides in length. Recent work has shown differential expression of mature micro-RNAs in human cancers. We characterized the alteration in expression of miR-29b in ovarian serous carcinoma. miR-29b expression was analyzed using quantitative stem-loop reverse transcriptase polymerase chain reaction on a set of 50 formal in-fixed, paraffin-embedded ovarian serous carcinoma samples. Protein expression of p53, estrogen receptor, progesterone receptor, human epidermal growth factor receptor 2, Ki-67, and insulinlike growth factor I was quantified in the corresponding tissue microarray. The expression profile of miR-29b was correlated with clinicopathological and patient survival data. We provide definitive evidence that miR-29b is down-regulated in a significant proportion of ovarian serous carcinomas and is associated with specific clinicopathological features, most notably high miR-29b expression being associated with reduced disease-free survival.