Spectroscopic studies on the interaction of pazufloxacin with calf thymus DNA.
Spectroscopic studies on the interaction of pazufloxacin with calf thymus DNA.
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DOI：
10.1016/j.jphotobiol.2008.07.008
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发表时间：
2008-12
期刊：
影响因子：
--
通讯作者：
Xia Ling;Wen-ying Zhong;Qi Huang;Kunyi Ni
中科院分区：
文献类型：
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作者：
Xia Ling;Wen-ying Zhong;Qi Huang;Kunyi Ni
采用荧光光谱、吸收光谱、DNA粘度滴定、竞争实验和碘化物猝灭实验研究DNA与帕珠沙星的相互作用。 DNA 显着猝灭帕珠沙星的荧光。紫外滴定实验中未观察到红移和等吸点。 DNA 粘度和碘化物猝灭结果表明帕珠沙星不会嵌入 DNA 中。 SsDNA 对帕珠沙星的猝灭作用比 dsDNA 更强。帕珠沙星与 DNA 的相互作用方式与溴化乙锭不同，溴化乙锭是一种典型的 DNA 嵌入剂。所有这些结果表明帕珠沙星与小牛胸腺DNA以沟槽结合的方式相互作用。还测定了淬火常数和热力学常数。
Fluorescence spectra, absorption spectra, DNA viscosity titrations, competition experiment, and iodide quenching experiment were used to study the interaction of DNA with pazufloxacin. DNA quenches the fluorescence of pazufloxacin significantly. No red shift and isobestic points are observed in UV titration experiment. DNA viscosity and iodide quenching results suggest that pazufloxacin does not intercalate into DNA. SsDNA has a stronger quenching effect on pazufloxacin than dsDNA has. Pazufloxacin interacts with DNA in a different mode from ethidium bromide, which is a typical intercalator of DNA. All these results indicate that pazufloxacin interacts with calf thymus DNA in the mode of groove binding. The quenching constant and thermodynamic constants have also been determined.