MicroRNA-210 promotes spinal cord injury recovery by inhibiting inflammation via the JAK-STAT pathway
MicroRNA-210 promotes spinal cord injury recovery by inhibiting inflammation via the JAK-STAT pathway
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DOI：
10.26355/eurrev_201810_16135
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发表时间：
2018-10-01
影响因子：
3.3
通讯作者：
Tang, X-M
中科院分区：
文献类型：
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作者：
Dai, J.;Yu, G-Y;Tang, X-M
目的：探讨microRNA-210在脊髓损伤（SCI）中的作用及其机制。材料与方法：建立小鼠脊髓损伤模型。将小鼠随机分为4组，分别为假手术组（sham组）、手术组（SCI组）、手术+NC组（SCI+NC组）和手术+microRNA-210过表达组（SCI+microRNA-210模拟组）。采用实时聚合酶链式反应（RT-PCR）检测四组患者不同时间点microRNA-210及JAK-STAT通路关键基因的mRNA表达水平。Western blot检测四组小鼠的JAK2和STAT3蛋白水平。为了研究microRNA-210在脊髓损伤恢复中的作用，我们检测了小鼠运动功能的变化。结果：假手术组小鼠左、右前肢握力在术后早期出现暂时性下降，术后第3天逐渐恢复到术前水平。而SCI组小鼠术后早期无法完成握力测定。脊髓损伤组小鼠术后第7天抓握能力恢复。此外，脊髓损伤组小鼠的握力显著低于假手术组，直到第50天结束。此外，SCI组小鼠的microRNA-210 mRNA水平呈时间依赖性下降(p
OBJECTIVE: To investigate the effect of microRNA-210 on the spinal cord injury (SCI) and its underlying mechanism.MATERIALS AND METHODS: The mouse SCI model was established. Mice were randomly assigned into 4 groups, namely the sham operation group (sham group), surgery group (SCI group), surgery+NC group (SCI+NC group) and surgery+microRNA-210 overexpression group (SCI+microRNA-210 mimics group). The mRNA levels of microRNA-210 and the key genes in the JAK-STAT pathway of the four groups were detected by Real-Time Polymerase Chain Reaction (RT-PCR) at different time points. Protein levels of JAK2 and STAT3 in mice of the four groups were detected by Western blot. To investigate the role of microRNA-210 in SCI recovery, changes in the motor function of mice were detected.RESULTS: Grip strengths of right and left forelimbs in mice from the sham group were temporarily decreased at the early stage after surgery, which were gradually recovered to the preoperative levels on the 3rd postoperative day. However, mice in SCI group were unable to complete the grip strength determination at the early stage after surgery. Mice in SCI group were capable of grasping on the 7th postoperative day. Besides, grip strengths of mice in SCI group were remarkably lower than those of sham group until the end-point (on the 50th day). Furthermore, mRNA levels of microRNA-210 in mice of SCI group were decreased in a time-dependent manner (p