Assessing the effects of adsorptive polymeric resin additions on fungal secondary metabolite chemical diversity.
Assessing the effects of adsorptive polymeric resin additions on fungal secondary metabolite chemical diversity.
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DOI：
10.1080/21501203.2014.942406
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发表时间：
2014-07-03
期刊：
影响因子：
--
通讯作者：
Tormo JR
中科院分区：
文献类型：
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作者：
González-Menéndez V;Asensio F;Moreno C;de Pedro N;Monteiro MC;de la Cruz M;Vicente F;Bills GF;Reyes F;Genilloud O;Tormo JR
吸附性聚合物树脂偶尔被描述为增强感兴趣的特定次级代谢产物（SM）的产生。在真菌发酵中诱导新化学实体表达的方法可能导致发现新的生物活性分子，并且应该作为创建用于药物先导发现的新微生物化学文库的可能工具来解决。在此，我们应用生物活性和化学评价来评估使用吸附树脂作为工具的差异表达的SM在真菌菌株集。数据自动化方法应用于提取物的超高效液相色谱分析，以评估在产生新的化学实体或改变在树脂和不同基础培养基存在下生长的真菌产生特定SM的一般影响。
Adsorptive polymeric resins have been occasionally described to enhance the production of specific secondary metabolites (SMs) of interest. Methods that induce the expression of new chemical entities in fungal fermentations may lead to the discovery of new bioactive molecules and should be addressed as possible tools for the creation of new microbial chemical libraries for drug lead discovery. Herein, we apply both biological activity and chemical evaluations to assess the use of adsorptive resins as tools for the differential expression of SMs in fungal strain sets. Data automation approaches were applied to ultra high performance liquid chromatography analysis of extracts to evaluate the general influence in generating new chemical entities or in changing the production of specific SMs by fungi grown in the presence of resins and different base media.