Analysis of a cellulose synthase catalytic subunit from the oomycete pathogen of crops Phytophthora capsici
Analysis of a cellulose synthase catalytic subunit from the oomycete pathogen of crops Phytophthora capsici
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作物辣椒疫霉卵菌病原体纤维素合酶催化亚基的分析
DOI：
10.1007/s10570-020-03362-9
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发表时间：
2020-08
期刊：
影响因子：
5.7
通讯作者：
Vincent Bulone
中科院分区：
文献类型：
--
作者：
Zhili Pang;Lauren S. McKe;Vaibhav Srivastava;Stefan Klinter;Sara M. Dı´az- Moreno;Peter Orlean;Xili Liu;Vincent Bulone
辣椒疫霉是一种重要的卵菌病原菌，每年造成重大的经济损失。它的细胞壁富含纤维素，对细胞的完整性和与宿主生物的相互作用至关重要。预计纤维素合成酶（CesA）蛋白质催化纤维素的聚合，但这还没有在卵菌的生化证明。在这里，我们提出了四个新鉴定的CesA蛋白质的性质从辣椒疫霉和比较其结构域组织与其他谱系的CesA。使用一个新构建的葡萄糖基转移酶缺陷的变种酿酒酵母低残留的背景活动，我们已经实现了成功的异源表达和生化特性的CesA蛋白从辣椒疫霉（PcCesA1）。我们的研究结果表明，个别PcCesA1酶产生纤维二糖作为主要的反应产物。免疫共沉淀研究和活性测定表明，几个PcCesA蛋白相互作用在一起，形成一个复杂的多蛋白质的性质是最有可能需要纤维素微纤维的形成。除了提供重要的见解纤维素合成的卵菌，我们的数据可能有助于长期识别细胞壁生物合成抑制剂，以控制感染的病原性卵菌。
Phytophthora capsici Leonian is an important oomycete pathogen of crop vegetables, causing significant economic losses each year. Its cell wall, rich in cellulose, is vital for cellular integrity and for interactions with the host organisms. Predicted cellulose synthase (CesA) proteins are expected to catalyze the polymerization of cellulose, but this has not been biochemically demonstrated in an oomycete. Here, we present the properties of the four newly identified CesA proteins from P. capsici and compare their domain organization with that of CesAs from other lineages. Using a newly constructed glucosyltransferase-deficient variant of Saccharomyces cerevisiae with low residual background activity, we have achieved successful heterologous expression and biochemical characterization of a CesA protein from P. capsici (PcCesA1). Our results demonstrate that the individual PcCesA1 enzyme produces cellobiose as the major reaction product. Co-immunoprecipitation studies and activity assays revealed that several PcCesA proteins interact together to form a complex whose multiproteic nature is most likely required for cellulose microfibril formation. In addition to providing important insights into cellulose synthesis in the oomycetes, our data may assist the longer term identification of cell wall biosynthesis inhibitors to control infection by pathogenic oomycetes.
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影响因子：
14.9
作者：
Tsirigos KD;Peters C;Shu N;Käll L;Elofsson A
通讯作者：
Elofsson A
DOI：
10.1073/pnas.87.20.8130
发表时间：
1990-10
影响因子：
11.1
作者：
Hing C. Wong;Anna Lisa Fear;Roger D. CALHOONt;Gregory H. Eichinger;Raphael Mayer;Dorit Amikam;Moshe Benziman;David H. Gelfand;J. H. Meade;W. Anne;Emerick;Robert Bruner;Arie BEN-BASSAT;R. Tal
通讯作者：
Hing C. Wong;Anna Lisa Fear;Roger D. CALHOONt;Gregory H. Eichinger;Raphael Mayer;Dorit Amikam;Moshe Benziman;David H. Gelfand;J. H. Meade;W. Anne;Emerick;Robert Bruner;Arie BEN-BASSAT;R. Tal
影响因子：
--
作者：
R. Gietz;R. A. Woods
通讯作者：
R. Gietz;R. A. Woods
DOI：
10.1073/pnas.0706569104
发表时间：
2007-09-25
影响因子：
11.1
作者：
Desprez, Thierry;Juraniec, Michal;Vernhettes, Samantha
通讯作者：
Vernhettes, Samantha
DOI：
10.1073/pnas.1606210113
发表时间：
2016-09
期刊：
Proceedings of the National Academy of Sciences
影响因子：
--
作者：
Pallinti Purushotham;Sung Hyun Cho;Sara M. Díaz-Moreno;Manish Kumar;B. Tracy Nixon;V. Bulone;J. Zimmer
通讯作者：
Pallinti Purushotham;Sung Hyun Cho;Sara M. Díaz-Moreno;Manish Kumar;B. Tracy Nixon;V. Bulone;J. Zimmer