Detection of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, swine influenza virus and Aujeszky's disease virus in cases of porcine proliferative and necrotizing pneumonia (PNP) in Spain
Detection of porcine reproductive and respiratory syndrome virus, porcine circovirus type 2, swine influenza virus and Aujeszky's disease virus in cases of porcine proliferative and necrotizing pneumonia (PNP) in Spain
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DOI：
10.1016/j.vetmic.2006.09.009
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发表时间：
2007-01-31
影响因子：
3.3
通讯作者：
Segales, Joaquim
中科院分区：
文献类型：
--
作者：
Grau-Roma, Llorenc;Segales, Joaquim
坏死性肺炎（PNP）是一种严重的间质性肺炎，其特征是2型肺细胞肥大和增殖，肺泡腔内存在坏死细胞。许多病毒因子与PNP病因学有关，特别强调猪繁殖与呼吸综合征病毒（PRRSV）。为了获得PNP因果关系的知识，对来自西班牙的74例断奶后猪的PNP病例进行了回顾性研究。结合组织病理学检查，原位杂交（ISH）和免疫组织化学（IHC）方法的PRRSV，猪流感病毒（SIV）和Aujeszky病病毒（ADV）的存在，猪圆环病毒2型（PCV 2）进行了研究。在PNP病例中，PCV 2是最流行的病毒因子（85.1%），其次是PRRSV（44.6%）; 39.1%的PNP病例显示PCV 2是唯一检测到的因子，而只有4.1%的病例将PRRSV作为唯一病原体。SIV和ADV在PNP病例中非常零星地检测到，并且总是与PCV 2合并感染。因此，目前的数据表明，PCV 2是西班牙PNP病例中最重要的病原体，而PRRSV对PNP的发展并不重要。考虑到所呈现的结果和现有文献，我们认为PCV 2可能是欧洲PNP病例的主要贡献者，而PRRSV可能在北美发挥类似的作用。(c)2006 Elsevier B. V.保留所有权利。
Proliferative and necrotizing pneumonia (PNP) is a severe form of interstitial pneumonia characterised by hypertrophy and proliferation of pneumocytes type 2 and presence of necrotic cells within alveoli lumen. Many viral agents have been linked to PNP aetiology, with especial emphasis on porcine reproductive and respiratory syndrome virus (PRRSV). To gain knowledge on PNP causality, a retrospective study on 74 PNP cases from postweaning pigs from Spain was carried out. Coupled with histopathological examinations, the presence of porcine circovirus type 2 (PCV2) by in situ hybridization (ISH), and PRRSV, swine influenza virus (SIV) and Aujeszky's disease virus (ADV) by immunohistochemical (IHC) methods, were investigated. PCV2 was the most prevalent viral agent in PNP cases (85.1%) followed by PRRSV (44.6%); 39.1% of PNP cases showed PCV2 as the solely detected agent, while only 4.1% had PRRSV as the unique pathogen. SIV and ADV were very sporadically detected in PNP cases, and always in co-infection with PCV2. Therefore, present data indicate that PCV2 is the most important aetiological agent in PNP cases from Spain and that PRRSV is not essential for the development of PNP. Taking into account the presented results and available literature, we suggest that PCV2 is possibly the main contributor to PNP cases in Europe while PRRSV could play a similar role in North America. (c) 2006 Elsevier B.V. All rights reserved.