Temperature-regulated model of focal ischemia in the mouse - A study with histopathological and behavioral outcomes
Temperature-regulated model of focal ischemia in the mouse - A study with histopathological and behavioral outcomes
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DOI：
10.1161/01.str.0000129653.22241.d7
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发表时间：
2004-07-01
期刊：
影响因子：
8.3
通讯作者：
Buchan, AM
中科院分区：
文献类型：
--
作者：
Barber, PA;Hoyte, L;Buchan, AM
背景和目的——随着基因操纵动物的发展，小鼠中风模型的重要性不断增加。我们假设缺血后立即低温可能会减轻小鼠的缺血性脑损伤。方法-将腹内射频探针植入动物体内并监测核心温度。组包括：MCAO-45-REG（45分钟大脑中动脉闭塞[MCAO]），缺血后温度控制>34℃，持续24小时；允许 MCAO-45（45 分钟 MCAO）在恢复期间自我调节核心温度； MCAO-30-REG（30 分钟 MCAO），与 MCAO-45-REG 具有相同的温度方案； MCAO-30（30 分钟 MCAO），温度方案与 MCAO-45 相同。第7天评估行为和组织学评分。对 18 个指定区域的损伤进行定性组织学评分评估。 结果 - 麻醉后前 4 小时，MCAO-45-REG 核心温度（平均 34.94°C+/-0.8°C）与自我调节（MCAO-45，平均 33.1°C+/-2.3°C）显着不同（P
Background and Purpose-The importance of mouse stroke models has increased with the development of genetically manipulated animals. We hypothesized that immediate postischemia hypothermia may attenuate ischemic brain injury in the mouse.Methods-Intraabdominal radio frequency probes were implanted in animals and core temperature monitored. Groups included: MCAO-45-REG (45 minutes middle cerebral artery occlusion [MCAO]) temperature-controlled in the postischemic period >34degreesC for 24 hours; MCAO-45 (45 minutes MCAO) were allowed to self-regulate core temperature during recovery; MCAO-30-REG (30 minutes MCAO), with the same temperature protocol as MCAO-45-REG; MCAO-30 (30 minutes MCAO), with temperature protocol the same as MCAO-45. Behavior and histological score was assessed at 7 days. The qualitative histological score assessed for injury in 18 specified regions.Results-MCAO-45-REG core temperature (mean 34.94degreesC+/-0.8degreesC) was significantly different than the self-regulating (MCAO-45, mean 33.1degreesC+/-2.3degreesC) for the first 4 hours after anesthesia (P