ISOLATION, PRIMARY STRUCTURE AND BIOACTIVITY OF SCHISTOFLRF-AMIDE, A FMRF-AMIDE-LIKE NEUROPEPTIDE FROM THE LOCUST, SCHISTOCERCA-GREGARIA
ISOLATION, PRIMARY STRUCTURE AND BIOACTIVITY OF SCHISTOFLRF-AMIDE, A FMRF-AMIDE-LIKE NEUROPEPTIDE FROM THE LOCUST, SCHISTOCERCA-GREGARIA
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DOI：
10.1016/0006-291x(89)92512-6
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发表时间：
1989-04-28
影响因子：
3.1
通讯作者：
EVANS, PD
中科院分区：
文献类型：
--
作者：
ROBB, S;PACKMAN, LC;EVANS, PD
我们使用基于高压液相色谱法的放射免疫纯化系统，从蝗虫（Schistocerca gregaria）的胸神经系统中分离出一种与Phe-Met-Arg-Phe-NH2家族相关的神经肽。该蝗虫肽的主要序列为pro - asp - val - asp - his - val - ph - leu - arg - ph - nh2。天然神经肽和合成神经肽的生物活性在蝗虫心脏和后肢伸胫肌的生物测定中是相同的。
We have isolated a neuropeptide, related to the Phe-Met-Arg-Phe-NH2 family, from the thoracic nervous system of the locust, Schistocerca gregaria, using a purification system based on the radioimmunoassay of high pressure liquid chromatography fractions. The primary sequence of this locust peptide is Pro-Asp-Val-Asp-His-Val-Phe-Leu-Arg-Phe-NH2. The bioactivities of the native and synthetic neuropeptide are identical in both the locust heart and hindling extensor-tibiae muscle bioassays.