Genetics of the white-opaque transition in Candida albicans: demonstration of switching recessivity and mapping of switching genes.
Genetics of the white-opaque transition in Candida albicans: demonstration of switching recessivity and mapping of switching genes.
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白色念珠菌白色-不透明转变的遗传学：转换隐性的演示和转换基因的定位。
DOI：
10.1128/jb.174.9.2951-2957.1992
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发表时间：
1992
影响因子：
3.2
通讯作者：
Magee,PT
中科院分区：
文献类型：
--
作者：
Chu,WS;Rikkerink,EH;Magee,PT
球形体融合已被用于分析白色念珠菌WO-1的可逆表型转变（白色-不透明）的遗传学。这种转变涉及细胞形状、渗透性和集落形态的变化。转换与非转换细胞的融合得到非转换融合子，表明白色不透明表型是隐性的。热激诱导的染色体丢失产生了融合子的分离子，这些分离子能够经历过渡，这表明阻遏物功能是遗传定义的，可能仅限于一条或两条染色体。染色体R、1、3、4和7作为阻遏物的独特位点被消除，留下2、5和6作为可能的位置。当ura 3（3号染色体）非转换菌株与转换菌株融合时，所有由热激诱导的ura 3分离子都不能进行表型转换。因此，一些或所有的基因（称为SWI基因）的转变是必不可少的位于染色体3。UV照射诱导的重组确实产生了Ura转换后代，表明转换失败不是由于嘧啶需求的副作用。未能隔离正常开关的紫外线照射产生的ura 3后代表明这两个基因之间的密切联系。
Spheroplast fusion has been used to analyze the genetics of the reversible phenotypic transition, white-opaque, in Candida albicans WO-1. This transition involves changes in cell shape, permeability, and colony morphology. Fusion of switching with nonswitching cells gave nonswitching fusants, suggesting that the white-opaque phenotype is recessive. Chromosome loss induced by heat shock gave segregants of the fusants which were able to undergo the transition, indicating that the repressor function is genetically defined and probably limited to one or two chromosomes. Chromosomes R, 1, 3, 4, and 7 were eliminated as unique sites for the repressor, leaving 2, 5, and 6 as possible locations. When a ura3 (chromosome 3) nonswitching strain was fused with a switching strain, all ura3 segregants induced by heat shock were incapable of the phenotypic transition. Therefore, some or all of the genes (called SWI genes) essential for the transition are located on chromosome 3. UV irradiation-induced recombination did give rise to Ura- switching progeny, showing that the failure to switch was not due to a side effect of the pyrimidine requirement. The failure to isolate normally switching ura3 progeny generated by UV irradiation suggests a close linkage between the two genes.