Aliphatic chain length by isotropic mixing (ALCHIM): determining composition of complex lipid samples by ¹H NMR spectroscopy.
Aliphatic chain length by isotropic mixing (ALCHIM): determining composition of complex lipid samples by ¹H NMR spectroscopy.
复制标题
通过各向同性混合测定脂肪链长度 (ALCHIM)：通过 1 H NMR 光谱测定复杂脂质样品的组成。
DOI：
10.1007/s10858-014-9836-0
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发表时间：
2014
影响因子：
2.7
通讯作者：
Conzen,SuzanneD
中科院分区：
文献类型：
--
作者：
Sachleben,JosephR;Yi,Ruiyang;Volden,PaulA;Conzen,SuzanneD
在医药、食品科学和生物化学等领域，对混合物中存在的脂类的数量和类型进行量化是很重要的。核磁共振波谱可以量化混合物中饱和和不饱和脂肪酸的总量，但通过核磁共振确定饱和脂肪酸的长度或不饱和脂肪酸的位置是一个艰巨的挑战。我们已经开发了一种核磁共振技术，通过各向同性混合来确定脂肪链的长度，以解决这个问题。用选择性全相关光谱技术激发和转移来自分辨共振的磁化强度，我们证明了这种转移到另一个分辨位置的时间依赖于分离两个位置的脂肪碳的数量线性地依赖于这两个位置。这项技术应用于复杂的天然混合物，允许识别和量化组成脂肪酸。该方法已被应用于整个脂肪细胞，表明它将在整个组织的研究中发挥重要作用。
Quantifying the amounts and types of lipids present in mixtures is important in fields as diverse as medicine, food science, and biochemistry. Nuclear magnetic resonance (NMR) spectroscopy can quantify the total amounts of saturated and unsaturated fatty acids in mixtures, but identifying the length of saturated fatty acid or the position of unsaturation by NMR is a daunting challenge. We have developed an NMR technique, aliphatic chain length by isotropic mixing, to address this problem. Using a selective total correlation spectroscopy technique to excite and transfer magnetization from a resolved resonance, we demonstrate that the time dependence of this transfer to another resolved site depends linearly on the number of aliphatic carbons separating the two sites. This technique is applied to complex natural mixtures allowing the identification and quantification of the constituent fatty acids. The method has been applied to whole adipocytes demonstrating that it will be of great use in studies of whole tissues.