Plant compounds that induce polychlorinated biphenyl biodegradation by Arthrobacter sp. strain B1B
Plant compounds that induce polychlorinated biphenyl biodegradation by Arthrobacter sp. strain B1B
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DOI：
10.1128/aem.63.5.1933-1938.1997
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发表时间：
1997-05-01
影响因子：
4.4
通讯作者：
Crowley, DE
中科院分区：
文献类型：
--
作者：
Gilbert, ES;Crowley, DE
以绿薄荷的化学成分4，4 '-二氯联苯环的裂解产物为基础，通过筛选试验，确定了能诱导节杆菌B1 B菌株共代谢多氯联苯（PCBs）的植物化合物（留兰香）、l-香芹酮诱导节杆菌属菌株BIB共代谢多氯联苯1242，导致混合物中26个峰的显著降解，包括选定的四氯联苯和五氯联苯。多氯联苯生物降解的证据包括峰值消失，形成的苯基hexadienoate环裂变产物，氯苯甲酸积累在培养上清液。香芹酮不被用作生长基质，并且在浓度大于500毫克/升时具有毒性（-1）结构上与l-香芹酮相关的几种化合物，包括柠檬烯、对伞花烃和异戊二烯，也可诱导节杆菌B1 B菌株对多氯联苯的共代谢。结构-活性分析表明，具有不饱和对薄荷烷结构基序的化学品促进最强的共代谢活性。这些数据表明，某些植物来源的萜类化合物可能是有用的，促进提高PCB的生物降解率土壤细菌。
Plant compounds that induced Arthrobacter sp, strain B1B to cometabolize polychlorinated biphenyls (PCBs) were identified by a screening assay based on the formation of a 4,4'-dichlorobiphenyl ring fission product, A chemical component of spearmint (Mentha spicata), l-carvone, induced Arthrobacter sp. strain BIB to cometabolize Aroclor 1242, resulting in significant degradation of 26 peaks in the mixture, including selected tetra- and pentachlorobiphenyls. Evidence for PCB biodegradation included peak disappearance, formation of a phenylhexadienoate ring fission product, and chlorobenzoate accumulation in the culture supernatant. Carvone was not utilized as a growth substrate and was toxic at concentrations of greater than 500 mg liter(-1) Several compounds structurally related to l-carvone, including limonene, p-cymene, and isoprene, also induced cometabolism of PCBs by Arthrobacer sp, strain B1B, A structure-activity analysis showed that chemicals with an unsaturated p-menthane structural motif promoted the strongest cometabolism activity. These data suggest that certain plant-derived terpenoids may be useful for promoting enhanced rates of PCB biodegradation by soil bacteria.