Biodegradation of naphthalene and anthracene by chemo-tactically active rhizobacteria of populus deltoides.

Biodegradation of naphthalene and anthracene by chemo-tactically active rhizobacteria of populus deltoides.
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DOI:
10.1590/s1517-838220100004000011
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发表时间:
2010-10
期刊:
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
影响因子:
--
通讯作者:
Bisht NS
Bisht NS
中科院分区:
其他
文献类型:
--
作者:
Bisht S;Pandey P;Sood A;Sharma S;Bisht NS

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从生长在非污染土壤中的美洲黑杨根际分离到几株降解菌。其中,库尔希氏菌、变种微球菌、耐辐射球菌和环状芽孢杆菌等4个菌株除利用菲和萘外,还利用大黄烯、苯、甲苯和二甲苯。库尔蒂亚氏菌和环状芽孢杆菌对萘和蒽的趋化反应呈阳性。随着底物浓度的增加(0.5~1.0 mg/50ml),菌株的平均生长速率常数(K)增大。环状芽孢杆菌SBA12和枯草杆菌SBA4对菲的降解率分别为87.5%和86.6%,而枯草杆菌则为87.5%和86.6%。高效液相分析表明,SBA4、环状芽孢杆菌SBA12和变种芽孢杆菌SBA8在培养6d后对萘的降解率分别为85.3%、95.8%和86.8%。
Several naphthalene and anthracene degrading bacteria were isolated from rhizosphere of Populus deltoides, which were growing in non-contaminated soil. Among these, four isolates, i.e. Kurthia sp., Micrococcus varians, Deinococcus radiodurans and Bacillus circulans utilized chrysene, benzene, toluene and xylene, in addition to anthracene and naphthalene. Kurthia sp and B. circulans showed positive chemotactic response for naphthalene and anthracene. The mean growth rate constant (K) of isolates were found to increase with successive increase in substrate concentration (0.5 to 1.0 mg/50ml). B. circulans SBA12 and Kurthia SBA4 degraded 87.5% and 86.6% of anthracene while, Kurthia sp. SBA4, B. circulans SBA12, and M. varians SBA8 degraded 85.3 %, 95.8 % and 86.8 % of naphthalene respectively after 6 days of incubation as determined by HPLC analysis.