Dihydropyrimidine dehydrogenase in normal and malignant endometrium: relationship with cell proliferation and thymidine phosphorylase
Dihydropyrimidine dehydrogenase in normal and malignant endometrium: relationship with cell proliferation and thymidine phosphorylase
复制标题
正常和恶性子宫内膜中的二氢嘧啶脱氢酶：与细胞增殖和胸苷磷酸化酶的关系
作者：
R. Fujiwaki;K. Iida;K. Nakayama;H. Kanasaki;T. Ozaki;K. Hata;E. Sakai;K. Miyazaki
二氢嘧啶脱氢酶(DPD)是一种嘧啶抢救酶，负责降解胸腺嘧啶，胸腺嘧啶核苷由胸腺嘧啶核苷磷酸化酶(TP)产生。本研究的目的是探讨DPD与子宫内膜细胞增殖和TP表达的关系。我们用逆转录聚合酶链式反应检测了58例正常子宫内膜和28例子宫内膜癌组织中DPD基因的表达，用酶联免疫吸附试验检测了DPD蛋白的水平，用免疫组织化学方法检测了DPD蛋白的定位。DPD基因表达与增殖细胞核抗原指数和TP基因表达相关。与DPD蛋白水平相关的DPD基因表达在月经各阶段相对稳定，但在绝经后显著升高。子宫内膜癌组明显低于正常子宫内膜组。定位分析表明，DPD蛋白主要定位于上皮细胞，但也存在于基质细胞。DPD基因表达与增殖细胞核抗原指数呈负相关。TP基因在绝经后子宫内膜和恶性子宫内膜中的表达模式与DPD的差异有统计学意义。在任何月经期，子宫内膜癌组织中TP/DPD基因的高比例表达均显著高于正常子宫内膜组织。DPD可能与TP协同作用，通过维持正常和恶性子宫内膜中的嘧啶核苷酸池平衡来影响细胞功能。
Dihydropyrimidine dehydrogenase (DPD) is a pyrimidine salvage enzyme responsible for degradation of thymine, which is produced from thymidine by thymidine phosphorylase (TP). Our purpose was to determine the relationship between DPD, cell proliferation and TP expression in human endometrium. We examined DPD gene expression using reverse transcription-polymerase chain reaction, DPD protein levels using enzyme-linked immunosorbent assay, and DPD protein localization using immunohistochemistry in 58 normal endometria and 28 endometrial cancers. DPD gene expression was then related to the proliferating cell nuclear antigen index and to TP gene expression. DPD gene expression, which was correlated with DPD protein level, was relatively stable throughout various menstrual phases but was significantly elevated in postmenopausal status. It was significantly lower in endometrial cancer than in normal endometrium. Localization analysis revealed that DPD protein was located primarily in epithelial cells, but was also present in stromal cells. DPD gene expression correlated inversely with the PCNA index. TP gene expression pattern contrasted with that of DPD in postmenopausal and malignant endometrium. A high ratio of TP to DPD gene expression was significantly more frequent in endometrial cancer than in normal endometrium in any menstrual phase. DPD may act cooperatively with TP to affect cell function by maintaining the pyrimidine nucleotide pool balance in normal and malignant endometrium.
使用 5-氟尿嘧啶作为底物，二氢尿嘧啶脱氢酶活性在人体组织中的分布和抑制。
影响因子：
2
作者：
Ho,DH;Townsend,L;Luna,MA;Bodey,GP
通讯作者：
Bodey,GP
正常和肿瘤人体组织中尿嘧啶分解代谢的酶。
影响因子：
11.2
作者：
Naguib,FN;elKouni,MH;Cha,S
通讯作者：
Cha,S
影响因子：
2.9
作者：
ILTZSCH, MH;ELKOUNI, MH;CHA, S
通讯作者：
CHA, S