Bone anabolic responses to mechanical load in vitro involve COX-2 and constitutive NOS
Bone anabolic responses to mechanical load in vitro involve COX-2 and constitutive NOS
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DOI：
10.1080/03008200490278133
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发表时间：
2004-01-01
影响因子：
2.9
通讯作者：
Stern, PH
中科院分区：
文献类型：
--
作者：
Kunnel, JG;Igarashi, K;Stern, PH
机械刺激对于维持包括骨骼在内的结缔组织的稳态和结构至关重要。我们研究的目的是测试几种潜在信号中间体在骨骼对新开发的微机械加载装置施加的负载的合成代谢反应中的重要性。从 7 至 8 天大的 CD-1 小鼠身上切下的胫骨以 1 Hz、1000 muepsilon（微应变）、峰值负载 100 mN 循环加载。通过分别测量 H-3-胸苷和 C-14-脯氨酸的掺入来评估 DNA 和蛋白质合成。通过在每种特定抑制剂存在下孵育骨骼来检查环氧合酶 (COX) 亚型、一氧化氮合酶 (NOS) 亚型和谷氨酸受体门控 Ca2+ 通道的作用。结果表明，COX-2和组成型NOS是新生儿胫骨对体外微机械负荷的合成代谢反应中的重要信号分子。
Mechanical stimulation is essential for maintaining the homeostasis and architecture of connective tissues including bone. The purpose of our study was to test the importance of several potential signaling intermediates in the anabolic responses of bone to loads applied with a newly developed micromechanical loading device. Tibial bones excised from 7- to 8-day-old CD-1 mice were cyclically loaded at 1 Hz, 1000 muepsilon (microstrain) at a peak load of 100 mN. DNA and protein synthesis were evaluated by measuring the incorporation of H-3-thymidine and C-14-proline, respectively. The roles of cyclooxygenase (COX) isoforms, nitric oxide synthase (NOS) isoforms, and glutamate receptor-gated Ca2+ channeling were examined by incubating the bones in the presence of each of their specific inhibitors. The results indicate that COX-2 and constitutive NOS are important signaling molecules in the anabolic responses of neonatal tibial bone to the micromechanical load in vitro.