Visualization of Toyoura sand-grown plant roots by X-ray computer tomography.
Visualization of Toyoura sand-grown plant roots by X-ray computer tomography.
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DOI：
10.5511/plantbiotechnology.20.0819a
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发表时间：
2020-11
影响因子：
1.6
通讯作者：
Y. Yoshida;T. Arita;J. Otani;S. Sawa
中科院分区：
文献类型：
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作者：
Y. Yoshida;T. Arita;J. Otani;S. Sawa
植物将根系建立为三维结构，利用根系探索土壤，吸收资源，提供机械锚固。简化的二维生长系统，如琼脂板，已被用于研究植物根系生物学的各个方面。然而，研究隐藏在不透明土壤中的更真实的根系三维结构和功能仍然是一个挑战。在这里，我们优化了基于x射线计算机断层扫描（CT）的完整根系可视化，使用Toyoura砂（一种用于岩土技术研究的标准硅砂）作为生长基质。根组织和Toyoura砂的x射线衰减密度明显，使得CT数据的图像分割清晰。高粱在土优拉沙地生长特别旺盛，可作为分析机械障碍对根系结构优化的模型。Toyoura砂的使用有可能将植物根系生物学和岩土技术应用联系起来。
Plants establish their root system as a three-dimensional structure, which is then used to explore the soil to absorb resources and provide mechanical anchorage. Simplified two-dimensional growth systems, such as agar plates, have been used to study various aspects of plant root biology. However, it remains challenging to study the more realistic three-dimensional structure and function of roots hidden in opaque soil. Here, we optimized X-ray computer tomography (CT)-based visualization of an intact root system by using Toyoura sand, a standard silica sand used in geotechnology research, as a growth substrate. Distinct X-ray attenuation densities of root tissue and Toyoura sand enabled clear image segmentation of the CT data. Sorghum grew especially vigorously in Toyoura sand and it could be used as a model for analyzing root structure optimization in response to mechanical obstacles. The use of Toyoura sand has the potential to link plant root biology and geotechnology applications.