Creation and characterization of a cell-death reporter cell line for hepatitis C virus infection
Creation and characterization of a cell-death reporter cell line for hepatitis C virus infection
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DOI：
10.1016/j.antiviral.2010.02.321
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发表时间：
2010-05-01
影响因子：
7.6
通讯作者：
Rice, Charles M.
中科院分区：
文献类型：
--
作者：
Chen, Zhilei;Simeon, Rudo L.;Rice, Charles M.
The present study describes the creation and characterization of a hepatoma cell line, n4mBid, that supports all stages of the hepatitis C virus (HCV) life cycle and strongly reports HCV infection by a cell-death phenotype. The n4mBid cell line is derived from the highly HCV-permissive Huh-7.5 hepatoma cell line and contains a modified Bid protein (mBid) that is cleaved and activated by the HCV serine protease NS3-4A. N4mBid exhibited a 10-20-fold difference in cell viability between the HCV-infected and mock-infected states, while the parental Huh-7.5 cells showed
