Curcumin-Silk Fibroin Nanoparticles for Enhanced Anti-Candida albicans Activity In Vitro and In Vivo
Curcumin-Silk Fibroin Nanoparticles for Enhanced Anti-Candida albicans Activity In Vitro and In Vivo
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姜黄素-丝素蛋白纳米颗粒增强体外和体内抗白色念珠菌活性
DOI：
10.1166/jbn.2019.2722
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发表时间：
2019-04-01
影响因子：
2.9
通讯作者：
Li, Xuefeng
中科院分区：
文献类型：
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作者：
Xue, Baiji;Zhang, Yuelan;Li, Xuefeng
姜黄素（Curcumin， CM）具有抗真菌等多种药理活性，但由于其在水介质中的溶解度低、生物利用度差、首过代谢广泛等特点，限制了其临床应用。我们旨在利用纳米技术解决CM的局限性，增强其抗真菌活性。以丝素（SF）为载体，采用超临界CO2 （SEDS）溶液增强分散技术制备了姜黄素-丝素纳米颗粒（CM-SF NPs）。采用扫描电镜（SEM）、傅里叶红外光谱（FTIR）、差示扫描量热仪（DSC）和热重仪（TGA）对CM-SF NPs进行表征。根据NPs的特征，我们在体外和体内评估了CM-SF NPs对白色念珠菌的抗真菌活性。通过SEDS建立了一种基于sf的给药体系（CM-SF NPs, 85 +/- 15 nm）。与原CM相比，CM- sf NPs的水溶性得到改善，抗真菌活性增强。天然化合物负载的SF纳米颗粒可能是治疗真菌感染的有前途的候选药物。
Curcumin (CM) has multiple pharmacological activities including anti-fungal activity, but its clinical application is limited due to low solubility in aqueous media, poor bioavailability and extensive first pass metabolism. We aimed to resolve the limitation and enhance antifungal activity of CM using nanotechnology. Using silk fibroin (SF) as a carrier, we fabricated curcumin-silk fibroin nanoparticles (CM-SF NPs) by solution enhanced dispersion of supercritical CO2 (SEDS) technique. The characterization of CM-SF NPs was analyzed using scanning electron microscopy (SEM), fourier transform infrared spectroscopy (FTIR), differential scanning calorimeter (DSC) and thermo gravimetric apparatus (TGA). Following characterization of the NPs, we evaluated the antifungal activity of CM-SF NPs against Candida albicans in vitro and in vivo. A SF-based drug delivery system (CM-SF NPs, 85 +/- 15 nm) was established by SEDS. Compared to original CM, water solubility of CM-SF NPs was improved, and its antifungal activity was enhanced. The natural compound-loaded SF nanoparticles may be a promising therapeutic candidate for fungal infection.