Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster
Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster
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DOI：
10.1111/j.1742-4658.2006.05504.x
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发表时间：
2006-11
期刊：
影响因子：
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通讯作者：
Tatsushi Ruike;R. Takeuchi;K. Takata;M. Oshige;N. Kasai;Kaori Shimanouchi;Y. Kanai;Ryo-ichi Nakamura;F. Sugawara;K. Sakaguchi
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文献类型：
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作者：
Tatsushi Ruike;R. Takeuchi;K. Takata;M. Oshige;N. Kasai;Kaori Shimanouchi;Y. Kanai;Ryo-ichi Nakamura;F. Sugawara;K. Sakaguchi
真核生物DNA聚合酶加工因子是增殖细胞核抗原，是DNA复制和修复机制的重要组成部分。在黑胃果蝇中，我们克隆了第二个不同于mus209基因编码的PCNA cDNA（为方便起见，本文将其称为DmPCNA1）。第二个PCNA cDNA （DmPCNA2）编码一个255个氨基酸的蛋白，与DmPCNA1同源性为51.7%，在果蝇发育过程中普遍表达。DmPCNA2在体内以三聚体形式定位于果蝇细胞核中，并与果蝇DNA聚合酶δ和ε相关。用甲基磺酸盐或过氧化氢处理细胞增加了与染色质相关的DmPCNA2和DmPCNA1的数量，而暴露于紫外线下只增加了DmPCNA1的相关水平。我们的观察结果表明，当DNA发生修复时，DmPCNA2可能作为DmPCNA1的独立滑动钳起作用。
The eukaryotic DNA polymerase processivity factor, proliferating cell nuclear antigen, is an essential component in the DNA replication and repair machinery. In Drosophila melanogaster, we cloned a second PCNA cDNA that differs from that encoded by the gene mus209 (for convenience called DmPCNA1 in this article). The second PCNA cDNA (DmPCNA2) encoded a 255 amino acid protein with 51.7% identity to DmPCNA1, and was ubiquitously expressed during Drosophila development. DmPCNA2 was localized in nuclei as a homotrimeric complex and associated with Drosophila DNA polymerase δ and εin vivo. Treatment of cells with methyl methanesulfonate or hydrogen peroxide increased the amount of both DmPCNA2 and DmPCNA1 associating with chromatin, whereas exposure to UV light increased the level of association of only DmPCNA1. Our observations suggest that DmPCNA2 may function as an independent sliding clamp of DmPCNA1 when DNA repair occurs.