Hemagglutination with Aldehyde-Fixed Erythrocytes for Assay of Antigens and Antibodies.∗
Hemagglutination with Aldehyde-Fixed Erythrocytes for Assay of Antigens and Antibodies.∗
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DOI：
10.3181/00379727-124-31953
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发表时间：
1967-04
影响因子：
--
通讯作者：
D. Bing;J. Weyand;A. Stavitsky
中科院分区：
文献类型：
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作者：
D. Bing;J. Weyand;A. Stavitsky
总结已经描述了通过戊二醛简单处理保存绵羊红细胞的方法。这些细胞可以在存在或不存在偶联试剂的情况下与蛋白质偶联，并被特异性抗蛋白血清特异性凝集。这些抗原缀合的细胞稳定至少6个月。利用这些细胞，发现血凝抑制是检测少量抗原的灵敏技术。作者感谢Weldon W.哈罗德感谢约翰·R博士。Murphy，西储大学医学院医学系，告知他们红细胞可以通过短暂暴露于戊二醛来固定。
Summary Methods have been described for the preservation of sheep erythrocytes by brief treatment with glutaraldehyde. These cells could be conjugated to proteins in the presence or absence of coupling reagents and were specifically agglutinated by specific anti-protein sera. These antigen-conjugated cells were stable for at least 6 months. Utilizing these cells, the inhibition of hemagglutination was found to be a sensitive technique for detection of small amounts of antigen. The authors appreciate the excellent technical assistance of Mr. Weldon W. Harold. They are grateful to Dr. John R. Murphy, Department of Medicine, Western Reserve University School of Medicine, for informing them that erythrocytes could be fixed by brief exposure to glutiaraldehyde.