Alginate oligomer induces nitric oxide (NO) production in RAW264.7 cells: elucidation of the underlying intracellular signaling mechanism.
Alginate oligomer induces nitric oxide (NO) production in RAW264.7 cells: elucidation of the underlying intracellular signaling mechanism.
复制标题
海藻酸盐低聚物诱导 RAW264.7 细胞中一氧化氮 (NO) 的产生：阐明潜在的细胞内信号传导机制。
DOI：
10.1080/09168451.2015.1052768
复制
发表时间：
2015
期刊：
影响因子：
--
通讯作者：
and Tatsuya Oda
中科院分区：
文献类型：
--
作者：
Mikinori Ueno;Kichul Cho;Satoru Nakazono;Shogo Isaka;Ryogo Abu;Satoshi Takeshita;Kenichi Yamaguchi;Daekyung Kim;and Tatsuya Oda
藻酸盐是一种具有免疫调节活性的酸性线性多糖。在这项研究中，我们发现酶消化的海藻酸盐低聚物（AO）与不同程度的聚合（DP; 2-5）诱导更高水平的一氧化氮（NO）的生产在RAW 264.7细胞比未消化的海藻酸盐聚合物（AP）。逆转录-聚合酶链反应和蛋白质印迹分析表明，诱导型NO合酶在AO处理的RAW 264. 7细胞中的表达水平高于AP处理的细胞。AO诱导RAW264.7细胞核因子（NF）-κB p65亚单位核转位的程度大于AP。虽然AO和AP在三种有丝分裂原活化蛋白（MAP）激酶中诱导了相似程度的磷酸化，但在三种MAP激酶抑制剂中，c-Jun N-末端激酶抑制剂对AO和AP处理的RAW264.7细胞中NO诱导表现出最有效的抑制作用。
Alginate is an acidic linear polysaccharide with immune-modulating activities. In this study, we found that enzymatically digested alginate oligomer (AO) with various degrees of polymerization (DP; 2–5) induced a higher level of nitric oxide (NO) production in RAW264.7 cells than undigested alginate polymer (AP). Reverse transcription-polymerase chain reaction and western blot analyses revealed that the expression level of inducible NO synthase in AO-treated RAW264.7 cells was higher than that in AP-treated cells. AO induced nuclear translocation of nuclear factor (NF)-κB p65 subunit in RAW264.7 cells to a greater extent than AP. Although AO and AP induced similar extents of phosphorylation in three mitogen-activated protein (MAP) kinases, c-Jun N-terminal kinase inhibitor exhibited the most potent inhibitory effect on NO induction in AO- and AP-treated RAW264.7 cells, among three MAP kinase inhibitors that were tested.