Qualitative and Quantitative Imaging Evaluation of Renal Cell Carcinoma Subtypes with Grating-based X-ray Phase-contrast CT.
Qualitative and Quantitative Imaging Evaluation of Renal Cell Carcinoma Subtypes with Grating-based X-ray Phase-contrast CT.
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DOI：
10.1038/srep45400
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发表时间：
2017-03-31
影响因子：
4.6
通讯作者：
Herzen J
中科院分区：
文献类型：
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作者：
Braunagel M;Birnbacher L;Willner M;Marschner M;De Marco F;Viermetz M;Notohamiprodjo S;Hellbach K;Auweter S;Link V;Woischke C;Reiser MF;Pfeiffer F;Notohamiprodjo M;Herzen J
目前的临床成像方法在检测和正确表征肾细胞癌（RCC）的不同亚型方面面临局限性，而这些对于治疗和预后非常重要。本研究评估了光栅X射线相衬计算机断层扫描（gbPC-CT）的可视化和表征人类RCC亚型的潜力。将23个离体福尔马林固定的人肾标本的成像结果与基于吸收的CT（gbCT）、临床CT和3 T MRI的结果进行比较，并使用组织学进行验证。将感兴趣区域置于每个标本上进行定量评价。gbPC-CT定性和定量成像可显著区分正常肾皮质（54 ± 4个HUP）与透明细胞（42 ± 10个）、乳头状（43 ± 6个）和嫌色细胞（39 ± 7个）RCC，p < 0.05。gbPC-CT、gbCT和临床CT检测肿瘤区域的灵敏度分别为100%、50%和40%。RCC结构如纤维束、假包膜、坏死或玻璃样变在gbPC-CT中被清楚地描绘，并且在gbCT、临床CT和MRI中不能同样良好地可视化。结果表明，gbPC-CT能够在不使用造影剂的情况下改善对正常肾实质和肿瘤组织以及RCC的不同软组织成分的区分。
Current clinical imaging methods face limitations in the detection and correct characterization of different subtypes of renal cell carcinoma (RCC), while these are important for therapy and prognosis. The present study evaluates the potential of grating-based X-ray phase-contrast computed tomography (gbPC-CT) for visualization and characterization of human RCC subtypes. The imaging results for 23 ex vivo formalin-fixed human kidney specimens obtained with phase-contrast CT were compared to the results of the absorption-based CT (gbCT), clinical CT and a 3T MRI and validated using histology. Regions of interest were placed on each specimen for quantitative evaluation. Qualitative and quantitative gbPC-CT imaging could significantly discriminate between normal kidney cortex (54 ± 4 HUp) and clear cell (42 ± 10), papillary (43 ± 6) and chromophobe RCCs (39 ± 7), p < 0.05 respectively. The sensitivity for detection of tumor areas was 100%, 50% and 40% for gbPC-CT, gbCT and clinical CT, respectively. RCC architecture like fibrous strands, pseudocapsules, necrosis or hyalinization was depicted clearly in gbPC-CT and was not equally well visualized in gbCT, clinical CT and MRI. The results show that gbPC-CT enables improved discrimination of normal kidney parenchyma and tumorous tissues as well as different soft-tissue components of RCCs without the use of contrast media.