Identification and characterization of microRNAs in white and brown alpaca skin.
Identification and characterization of microRNAs in white and brown alpaca skin.
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DOI：
10.1186/1471-2164-13-555
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发表时间：
2012-10-16
期刊：
影响因子：
4.4
通讯作者：
Dong C
中科院分区：
文献类型：
--
作者：
Tian X;Jiang J;Fan R;Wang H;Meng X;He X;He J;Li H;Geng J;Yu X;Song Y;Zhang D;Yao J;Smith GW;Dong C
MicroRNA (miRNA) 是小的非编码 21-25 nt RNA 分子，在调节基因表达中发挥重要作用。关于皮肤中 miRNA 的表达谱和功能及其在色素沉着中的作用，人们知之甚少。羊驼有超过 22 种天然毛色，比任何其他产生纤维的物种都多。为了更好地了解 miRNA 在控制毛色中的作用，我们对白色羊驼和棕色羊驼皮肤中的 miRNA 表达谱进行了全面分析。借助 Illumina 测序技术对来自白色羊驼 (WA) 和棕色羊驼 (BA) 皮肤的两个小 RNA 文库进行了测序。分别从 WA 和 BA 皮肤文库中获得 272 个和 267 个保守 miRNA。在这些保守的 miRNA 中，WA 和 BA 皮肤中分别有 35 个和 13 个更丰富。根据基因本体论和 KEGG 通路分析对这些 miRNA 的靶标进行预测和分组。这些 miRNA 的许多预测靶基因都参与控制色素沉着的黑素生成途径。除了保守的 miRNA 之外，我们还从 WA 和 BA 皮肤库中获得了 22 个潜在的新颖 miRNA。这项研究首次通过深度测序分析对不同毛色动物皮肤中表达的 miRNA 进行了全面调查。我们发现了一系列在 WA 和 BA 皮肤中差异表达的 miRNA。结果表明 miRNA 在毛色调节中具有重要的潜在功能。
MicroRNAs (miRNAs) are small, non-coding 21–25 nt RNA molecules that play an important role in regulating gene expression. Little is known about the expression profiles and functions of miRNAs in skin and their role in pigmentation. Alpacas have more than 22 natural coat colors, more than any other fiber producing species. To better understand the role of miRNAs in control of coat color we performed a comprehensive analysis of miRNA expression profiles in skin of white versus brown alpacas. Two small RNA libraries from white alpaca (WA) and brown alpaca (BA) skin were sequenced with the aid of Illumina sequencing technology. 272 and 267 conserved miRNAs were obtained from the WA and BA skin libraries, respectively. Of these conserved miRNAs, 35 and 13 were more abundant in WA and BA skin, respectively. The targets of these miRNAs were predicted and grouped based on Gene Ontology and KEGG pathway analysis. Many predicted target genes for these miRNAs are involved in the melanogenesis pathway controlling pigmentation. In addition to the conserved miRNAs, we also obtained 22 potentially novel miRNAs from the WA and BA skin libraries. This study represents the first comprehensive survey of miRNAs expressed in skin of animals of different coat colors by deep sequencing analysis. We discovered a collection of miRNAs that are differentially expressed in WA and BA skin. The results suggest important potential functions of miRNAs in coat color regulation.