Argosomes: A potential vehicle for the spread of morphogens through epithelia
Argosomes: A potential vehicle for the spread of morphogens through epithelia
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DOI：
10.1016/s0092-8674(01)00484-6
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发表时间：
2001-09-07
期刊：
影响因子：
64.5
通讯作者：
Eaton, S
中科院分区：
文献类型：
--
作者：
Greco, V;Hannus, M;Eaton, S
形态发生素梯度的形成对于组织图案化至关重要。形态发生素从生产细胞中释放并扩散到邻近组织；然而，矛盾的是，许多形态发生素，包括 Wingless，与细胞膜紧密结合。在这里，我们描述了一种新颖的细胞生物学机制，该机制可通过果蝇成虫盘上皮将膜碎片长距离分散。我们将这些膜外囊泡称为细胞核体。细胞核体源自基底外侧膜，由椎间盘的许多不同区域产生。它们穿过邻近组织，主要存在于核内体中。 Wingless 蛋白与来自 Wingless 产生细胞的细胞核共定位。细胞核体的特性与它们作为 Wingless 蛋白传播的载体是一致的。
The formation of morphogen gradients is essential for tissue patterning. Morphogens are released from producing cells and spread through adjacent tissue; paradoxically, however, many morphogens, including Wingless, associate tightly with the cell membrane. Here, we describe a novel cell biological mechanism that disperses membrane fragments over large distances through the Drosophila imaginal disc epithelium. We call these membrane exovesicles argosomes. Argosomes are derived from basolateral membranes and are produced by many different regions of the disc. They travel through adjacent tissue where they are found predominantly in endosomes. Wingless protein colocalizes with argosomes derived from Wingless-producing cells. The properties of argosomes are consistent with their being a vehicle for the spread of Wingless protein.