The Cotton miR477-CBP60A Module Participates in Plant Defense Against Verticillium dahlia
The Cotton miR477-CBP60A Module Participates in Plant Defense Against Verticillium dahlia
复制标题
棉花 miR477-CBP60A 模块参与植物防御大丽黄萎病
DOI：
10.1094/mpmi-10-19-0302-r
复制
发表时间：
2020-04-01
影响因子：
3.5
通讯作者：
Wu, Jiahe
中科院分区：
文献类型：
--
作者：
Hu, Guang;Hao, Mengyan;Wu, Jiahe
先前的报道表明，当大丽轮枝菌定位于根表面时，在早期诱导阶段鉴定出了许多微小RNA（miRNA）。在此，我们根据棉花根在后期诱导阶段（此时病原体定位于根组织内部）对大丽轮枝菌响应的两个时间点的小RNA（sRNA）构建了两组。我们从病原体诱导的和对照的sRNA的六个文库中鉴定出71种已知的和378种新的miRNA。结合降解组和sRNA测序，鉴定出178个相应的miRNA靶基因，其中来自差异表达miRNA的40个靶基因主要与氧化还原和应激反应相关。更重要的是，我们对棉花在植物对大丽轮枝菌感染后期响应中的miR477 - CBP60A模块进行了表征。β - 葡萄糖醛酸苷酶融合报告基因和切割位点分析表明，ghr - miR477在转录后过程中直接切割GhCBP60A的信使RNA。ghr - miR477沉默降低了植物对这种真菌的抗性，而GhCBP60A的敲低则增加了植物抗性，其通过调节GhICS1的表达来决定水杨酸水平。我们的数据记录了植物对大丽轮枝菌响应中众多后期诱导的miRNA，表明这些miRNA在植物对维管束病害的抗性中起重要作用。
Previous reports have shown that, when Verticillium dahliae localizes at the root surface, many microRNAs (miRNAs) were identified at the early induction stage. Here, we constructed two groups from two timepoints of small RNA (sRNA) in cotton root responses to V. dahliae at the later induction stage, pathogen localizing in the interior of root tissue. We identified 71 known and 378 novel miRNAs from six libraries of the pathogen-induced and the control sRNAs. Combined with degradome and sRNA sequencing, 178 corresponding miRNA target genes were identified, in which 40 target genes from differentially expressed miRNAs were primarily associated with oxidation-reduction and stress responses. More importantly, we characterized the cotton miR477-CBP60A module in the later response of the plant to V. dahliae infection. A b-glucuronidase fusion reporter and cleavage site analysis showed that ghr-miR477 directly cleaved the messenger RNA of GhCBP60A in the posttranscriptional process. The ghrmiR477-silencing decreased plant resistance to this fungus, while the knockdown of GhCBP60A increased plant resistance, which regulated GhICS1 expression to determine salicylic acid level. Our data documented that numerous later-inducible miRNAs in the plant response to V. dahliae, suggesting that these miRNAs play important roles in plant resistance to vascular disease.