Surfactant protein a polymorphism is associated with susceptibility to chronic obstructive pulmonary disease in Chinese Uighur population
Surfactant protein a polymorphism is associated with susceptibility to chronic obstructive pulmonary disease in Chinese Uighur population
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表面活性蛋白a多态性与中国维吾尔族人群慢性阻塞性肺疾病易感性相关
DOI：
10.1007/s11596-012-0033-7
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发表时间：
2012-04-01
影响因子：
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通讯作者：
Xu, Yongjian
中科院分区：
文献类型：
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作者：
Guan, Jian;Liu, Xiansheng;Xu, Yongjian
本研究探讨表面活性蛋白-A(SP-A)基因多态性与新疆维吾尔族慢性阻塞性肺疾病(COPD)易感性的关系。从194名COPD吸烟者和201名健康的维吾尔族吸烟者的外周血中提取基因组DNA，这些人在2009年3月至2010年12月期间在四家新疆医院中的一家住院或就诊。研究了SP-A基因aa62(CCA/CCG rs1136451)和aa219(CGG/TGG，rs4253527)的单核苷酸多态性。用TaqMan聚合酶链式反应(PCR)检测基因分型。结果显示，慢性阻塞性肺疾病患者与正常吸烟者Aa62基因的基因频率存在差异(x2=6.852，P=0.033)。等位基因频率与对照组比较差异有统计学意义(x2=6.545，P=0.011；OR=0.663；95%CI：0.484~0.909)。提示SP-A基因aa62(CCA/CCG，rs1136451)多态性可能与新疆维吾尔族COPD易感性有关。
This study investigated the correlation between surfactant protein-A (SP-A) polymorphism and the susceptibility of chronic obstructive pulmonary disease (COPD) in Xinjiang Uighurs. Genomic DNA was extracted from peripheral blood of 194 COPD smokers and 201 healthy smokers of Uighur who were hospitalized in or paid a visit to one of the four Xinjiang-based hospitals involved in the study, from March 2009 to December 2010. Single nucleotide polymorphisms (SNPs) were studied at aa62 (CCA/CCG rs1136451) and aa219 (CGG/TGG, rs4253527) in SP-A. Genotypes were determined by using the TaqMan polymerase chain reaction (PCR). Our results showed that genotype frequencies were different between the COPD and normal smokers in aa62 (x2=6.852,P=0.033). There were also significant differences in allele genotype frequencies between the COPD and the control and allele G might decrease the risk COPD (x2=6.545,P=0.011; OR=0.663; 95% CI: 0.484–0.909). The result suggested that polymorphism of aa62 (CCA/CCG, rs1136451) of SP-A may be associated with the susceptibility to COPD in Xinjiang Uighurs.