L-Sulforaphane Confers Protection Against Oxidative Stress in an In Vitro Model of Age-Related Macular Degeneration
L-Sulforaphane Confers Protection Against Oxidative Stress in an In Vitro Model of Age-Related Macular Degeneration
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DOI：
10.2174/1874467211666180125163009
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发表时间：
2018-01-01
影响因子：
2.7
通讯作者：
Kwa, Faith A. A.
中科院分区：
文献类型：
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作者：
Dulull, Nabeela K.;Dias, Daniel A.;Kwa, Faith A. A.
背景资料：在年龄相关性黄斑变性中，视网膜中的氧化损伤和异常新生血管形成是由血管内皮生长因子的上调和谷胱甘肽-S-转移酶基因的表达降低引起的。目前的治疗只能治标不治本。十字花科蔬菜中的化合物目的：研究L-萝卜硫素作为改善老年性黄斑变性（age-related macular degeneration，AMD）中异常基因表达和代谢物水平的潜在治疗药物。方法：AMD的体外氧化应激模型是基于将成人视网膜色素上皮细胞系暴露于200 μ M过氧化氢。MTS法检测L-萝卜硫素对细胞增殖的影响。GSTM 1，VEGFA，DNMT 1和HDAC 6基因在调节这些影响的作用进行了研究，使用定量实时聚合酶链反应。通过气相色谱-质谱法建立了L-萝卜硫素处理的细胞的代谢谱。使用单因素方差分析、学生t检验和事后Bonferroni统计检验验证对照组和治疗组之间的显著差异（p
Background: In age-related macular degeneration, oxidative damage and abnormal neovascularization in the retina are caused by the upregulation of vascular endothelium growth factor and reduced expression of Glutathione-S-transferase genes. Current treatments are only palliative. Compounds from cruciferous vegetables (e.g. L-Sulforaphane) have been found to restore normal gene expression levels in diseases including cancer via the activity of histone deacetylases and DNA methyl-transferases, thus retarding disease progression.Objective: To examine L-Sulforaphane as a potential treatment to ameliorate aberrant levels of gene expression and metabolites observed in age-related macular degeneration.Method: The in vitro oxidative stress model of AMD was based on the exposure of Adult Retinal Pigment Epithelium-19 cell line to 200 mu M hydrogen peroxide. The effects of L-Sulforaphane on cell proliferation were determined by MTS assay. The role of GSTM1, VEGFA, DNMT1 and HDAC6 genes in modulating these effects was investigated using quantitative real-time polymerase chain reaction. The metabolic profiling of L-Sulforaphane-treated cells via gas-chromatography mass-spectrometry was established. Significant differences between control and treatment groups were validated using one-way ANOVA, student t-test and post-hoc Bonferroni statistical tests (p