Cellular and molecular mechanisms of border cell migration analyzed using time-lapse live-cell Imaging
Cellular and molecular mechanisms of border cell migration analyzed using time-lapse live-cell Imaging
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DOI：
10.1016/j.devcel.2007.03.021
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发表时间：
2007-06-01
影响因子：
11.8
通讯作者：
Montell, Denise J.
中科院分区：
文献类型：
--
作者：
Prasad, Mohit;Montell, Denise J.
果蝇卵巢中的边缘细胞起源于上皮细胞，从上皮细胞中分离出来，侵入邻近的滋养细胞，并作为一个连贯的簇迁移。这种迁移已成为了解上皮细胞运动性的一个有用的遗传模型。生长因子介导的趋化性的普遍模型，特别是边界细胞，假定受体激活促进细胞突起的前沿。在这里，我们报告了边界细胞迁移的延时视频成像，使我们能够测试这个模型。降低导向受体EGFR和PVR的活性并未导致预期的突出抑制，而是导致所有方向的突出。相比之下，Notch活性的降低导致细胞无法从上皮细胞脱离而不影响方向感测。这些观察结果提供了新的见解，在体内细胞迁移的细胞动力学和分子机制。
Border cells in the Drosophila ovary originate within an epithelium, detach from it, invade neighboring nurse cells, and migrate as a coherent cluster. This migration has served as a useful genetic model for understanding epithelial cell motility. The prevailing model of growth factor-mediated chemotaxis in general, and of border cells in particular, posits that receptor activation promotes cellular protrusion at the leading edge. Here we report the time-lapse video imaging of border cell migration, allowing us to test this model. Reducing the activities of the guidance receptors EGFR and PVR did not result in the expected inhibition of protrusion, but instead resulted in protrusion in all directions. In contrast, reduction in Notch activity resulted in failure of the cells to detach from the epithelium without affecting direction sensing. These observations provide new insight into the cellular dynamics and molecular mechanisms of cell migration in vivo.