Rapid chromatofocusing of proteins.
Rapid chromatofocusing of proteins.
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蛋白质的快速色谱聚焦。
DOI：
10.1016/0003-2697(82)90105-1
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发表时间：
1982
影响因子：
2.9
通讯作者：
Regnier,FE
中科院分区：
文献类型：
--
作者：
Wagner,G;Regnier,FE
色谱聚焦是一种色谱技术，根据其pI值从离子交换载体上选择性洗脱蛋白质，已适用于高效液相色谱。结果发现，色谱聚焦可以检测蛋白质制剂中的异质性，而不是通过反相或尺寸排阻色谱证明，色谱聚焦的分辨率与离子交换色谱相当。虽然层析聚焦可能不能像等电聚焦那样分辨蛋白质，但它在速度和容量上都优于这种技术。讨论了色谱聚焦作为蛋白质分析和制备中的一种附加技术的实用性。本文描述的快速分离技术能够在约30分钟内以色谱聚焦模式解析蛋白质混合物。
Chromatofocusing, a chromatographic technique whereby proteins are selectively eluted from an ion-exchange support according to their pI values, has been adapted to high-performance liquid chromatography. It was found that chromatofocusing can detect heterogeneity in protein preparations not demonstrated by reverse-phase or size exclusion chromatography and that the resolution of chromatofocusing is comparable to ion-exchange chromatography. Although chromatofocusing may not resolve proteins as well as isoelectric focusing, it has advantages over this technique in both speed and capacity. The usefulness of chromatofocusing as an additional technique in the analysis and preparation of proteins is discussed. The rapid separation technique described here is able to resolve protein mixtures in the chromatofocusing mode in approximately 30 min.