Comparison of the inhibition of monoamine oxidase and butyrylcholinesterase activities by infusions from green tea and some citrus peels.
Comparison of the inhibition of monoamine oxidase and butyrylcholinesterase activities by infusions from green tea and some citrus peels.
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DOI：
10.1155/2014/586407
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发表时间：
2014-01-01
影响因子：
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通讯作者：
Oboh, Ganiyu
中科院分区：
文献类型：
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作者：
Ademosun, Ayokunle O;Oboh, Ganiyu
本研究旨在研究从绿色茶（茶树）和一些柑橘皮[柚子（柑橘），葡萄柚（柑橘天堂），和橙子（柑橘）]输注对神经退行性疾病的管理相关的关键酶[单胺氧化酶（MAO）和丁酰胆碱酯酶（BChE）]的影响。以2，2（'）-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）自由基清除能力、铁还原抗氧化能力和铁螯合能力为代表，研究了它们的总酚含量和抗氧化活性。绿色茶的总酚和总黄酮含量最高，分别为43.3mg/g和16.4mg/g，其次是橙子[总酚19.6mg/g，总黄酮6.5mg/g]、柚子[总酚16.3mg/g，总黄酮5.2mg/g]和柚子[总酚17.7mg/g，总黄酮5.9mg/g]。橙子对单胺氧化酶的抑制作用最强（EC 50 = 1.78mg/mL），而绿色茶对单胺氧化酶的抑制作用最弱（EC 50 = 2.56mg/mL）。绿色茶对BChE的抑制作用最小（EC_（50）= 5.43mg/mL）。而绿色茶浸液的ABTS自由基清除能力、还原力和Fe（2+）螯合能力最强。通过绿色茶和柑橘皮输注液抑制MAO和BChE活性，可以使它们成为预防/管理神经退行性疾病的良好饮食手段。
This study sought to investigate the effect of infusions from green tea (Camellia sinensis) and some citrus peels [shaddock (Citrus maxima), grapefruit (Citrus paradisi), and orange (Citrus sinensis)] on key enzymes relevant to the management of neurodegenerative conditions [monoamine oxidase (MAO) and butyrylcholinesterase (BChE)]. The total phenol contents and antioxidant activities as typified by their 2,2(')-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radicals scavenging abilities, ferric reducing antioxidant properties, and Fe(2+) chelating abilities were also investigated. Green tea had the highest total phenol (43.3mg/g) and total flavonoid (16.4mg/g) contents, when compared to orange [total phenol (19.6mg/g), total flavonoid (6.5mg/g)], shaddock [total phenol (16.3mg/g), total flavonoid (5.2mg/g)], and grapefruit [total phenol (17.7mg/g), total flavonoid (5.9mg/g)]. Orange (EC50 = 1.78mg/mL) had the highest MAO inhibitory ability, while green tea had the least MAO inhibitory ability (EC50 = 2.56mg/mL). Similarly, green tea had the least BChE inhibitory ability (EC50 = 5.43mg/mL) when compared to the citrus peels' infusions. However, green tea infusions had the strongest highest ABTS radical scavenging ability, reducing power, and Fe(2+) chelating ability. The inhibition of MAO and BChE activities by the green tea and citrus peels infusions could make them good dietary means for the prevention/management of neurodegenerative conditions.