Ceramide and sphingomyelinases in the regulation of stress responses
Ceramide and sphingomyelinases in the regulation of stress responses
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DOI：
10.1016/s0009-3084(99)00082-1
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发表时间：
1999-11-01
影响因子：
3.4
通讯作者：
Hannun, YA
中科院分区：
文献类型：
--
作者：
Sawai, H;Hannun, YA
神经酰胺和其他鞘脂是目前公认的新型细胞内信号介质。鞘脂代谢中的一个重要且受调节的步骤是鞘磷脂酶将鞘磷脂水解成神经酰胺。尽管一些研究表明酸性鞘磷脂酶在细胞调节中的作用，但几条研究线表明中性鞘磷脂酶（N-SMase）在包括细胞凋亡的应激反应中起关键作用。最近报道了大鼠脑中性膜镁依赖性神经鞘磷脂酶的进一步纯化，纯化的N-SMase比活性比大鼠脑匀浆提高约3000倍，它能被磷脂酰丝氨酸特异性激活。在细胞中，N-SMase可以与氧化还原状态和/或谷胱甘肽代谢偶联。本文还讨论了N-SMase和神经酰胺在应激反应中的意义。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
Ceramide and other sphingolipids are now recognized as novel intracellular signal mediators. One of the important and regulated steps in the metabolism of sphingolipids is the hydrolysis of sphingomyelin into ceramide by sphingomyelinases. Whereas some studies suggest a role for acid sphingomyelinase in cell regulation, several lines of investigation suggest that neutral sphingomyelinase (N-SMase) plays a critical role in stress responses including apoptosis. Recently the advanced purification of neutral membrane-bound magnesium-dependent sphingomyelinase from rat brain was reported on. The specific activity of the purified N-SMase was increased by approximately 3000-fold over the rat brain homogenate, and it is specifically activated by phosphatidylserine. In cells, N-SMase may be coupled to either the redox state and/or glutathione metabolism. The significance of N-SMase and ceramide in stress responses is discussed. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.