Rapid classical conditioning of odor response in a physiological model for olfactory research, the tiger salamander.
Rapid classical conditioning of odor response in a physiological model for olfactory research, the tiger salamander.
复制标题
嗅觉研究生理模型虎蝾螈中气味反应的快速经典调节。
DOI：
10.1093/chemse/22.3.277
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发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
Kauer,JS
中科院分区：
文献类型：
--
作者：
Dorries,KM;White,J;Kauer,JS
近年来，在理解与嗅觉功能相关的细胞机制方面取得了许多重要进展。然而，由于缺乏关于同一实验物种刺激辨别的心理物理学数据，这些发现产生的关于气味结构、生理活动和行为结果之间复杂关系的假设在很大程度上仍未得到检验。行为和生理反应之间的比较对于阐明感觉系统中刺激编码的关键方面至关重要。我们开发了一种方法，用于在嗅觉研究中使用的主要模型物种之一虎蝾螈（Ambystoma tigrinum）中生成心理物理数据。这些心理物理实验是在与我们实验室的生理实验相同的条件下进行的。使用经典调理，对个体蝾螈进行 2-3 小时的训练，以显示皮肤对气味而不是空气的潜在反应。未能使用后向配对进行训练表明该反应不是由于敏化或伪条件作用所致。条件反应是由嗅觉通路介导的，因为它被嗅觉神经部分阻断。我们发现蝾螈能够检测到生理实验中常用的三种气味剂（丁醇、乙酸丁酯和乙酸戊酯），但无法检测到第四种常见的实验气味剂——樟脑。该方法应该是通过提供蝾螈生理研究中使用的刺激可辨别性的数据来研究嗅觉信息处理的强大工具。化学。 《感官 22》：277–286，1997。
In recent years there have been a number of important advances in the understanding of cellular mechanisms related to olfactory function. Hypotheses regarding the complex relationships among odorant structure, physiological activity and behavioral outcome generated by these findings, however, remain largely untested due to a paucity of psychophysical data on stimulus discrimination in the same experimental species. Comparisons between behavioral and physiological responses are essential for elucidating the critical aspects of stimulus coding in sensory systems. We have developed a method for generating psychophysical data in one of the primary model species used in olfactory research, the tiger salamander,Ambystoma tigrinum. These psychophysical experiments are carried out under the same conditions as physiological experiments in our laboratory. Using classical conditioning, individual salamanders are trained over a period of 2–3 h to show skin potential responses to odor and not air. Failure to train using backward pairing demonstrates that the response is not due to sensitization or pseudoconditioning. The conditioned response is mediated by the olfactory pathway, as it is blocked by olfactory nerve section. We show that salamanders detect three odorants that are commonly used stimuli in physiological experiments (butyl alcohol, butyl acetate and amyl acetate), but cannot detect a fourth common experimental odorant, camphor. This method should be a powerful tool for studying olfactory information processing by providing data on discriminability of stimuli used in salamander physiological studies.Chem. Senses 22: 277–286, 1997.