Identification of three putative signal transduction genes involved in R gene-specified disease resistance in Arabidopsis.
Identification of three putative signal transduction genes involved in R gene-specified disease resistance in Arabidopsis.
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发表时间：
1999-05
期刊：
影响因子：
3.3
通讯作者：
Randall F. Warren;Peter M. Merritt;E. B. Holub;R. Innes
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文献类型：
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作者：
Randall F. Warren;Peter M. Merritt;E. B. Holub;R. Innes
拟南芥的RPS5抗病基因介导了对具有无毒基因avrPphB的丁香假单胞菌菌株的识别。通过筛选rps5特异性抗性缺失，我们鉴定出5个pbs （avrPphB易感）突变体，它们代表3种不同的基因。PBS1突变完全阻断了rps5介导的抗性，但对其他抗病基因指定的抗性几乎没有影响，这表明PBS1促进了avrPphB蛋白的识别。pbs2突变显著降低了RPS5和RPM1抗性基因介导的抗性，但对RPS4介导的抗性无明显影响，对rps2介导的抗性有中间作用。pbs2突变对7种不同的Peronospora parasitica识别基因介导的抗性也有不同的影响。这些数据表明，PBS2蛋白在仅对一部分抗病基因重要的途径中起作用。pbs3突变部分抑制了4个丁香假单胞菌耐药基因（RPS5、RPM1、RPS2和RPS4），对RPP基因有弱到中等的影响。此外，pbs3突变体在对丁香假单胞菌的毒力反应中允许更高的细菌生长，这表明pbs3基因产物在限制毒力和无毒病原体传播的途径中起作用。pbs突变是隐性的，已被定位到染色体I （pbs2）和V （pbs1和pbs3）。
The RPS5 disease resistance gene of Arabidopsis mediates recognition of Pseudomonas syringae strains that possess the avirulence gene avrPphB. By screening for loss of RPS5-specified resistance, we identified five pbs (avrPphB susceptible) mutants that represent three different genes. Mutations in PBS1 completely blocked RPS5-mediated resistance, but had little to no effect on resistance specified by other disease resistance genes, suggesting that PBS1 facilitates recognition of the avrPphB protein. The pbs2 mutation dramatically reduced resistance mediated by the RPS5 and RPM1 resistance genes, but had no detectable effect on resistance mediated by RPS4 and had an intermediate effect on RPS2-mediated resistance. The pbs2 mutation also had varying effects on resistance mediated by seven different RPP (recognition of Peronospora parasitica) genes. These data indicate that the PBS2 protein functions in a pathway that is important only to a subset of disease-resistance genes. The pbs3 mutation partially suppressed all four P. syringae-resistance genes (RPS5, RPM1, RPS2, and RPS4), and it had weak-to-intermediate effects on the RPP genes. In addition, the pbs3 mutant allowed higher bacterial growth in response to a virulent strain of P. syringae, indicating that the PBS3 gene product functions in a pathway involved in restricting the spread of both virulent and avirulent pathogens. The pbs mutations are recessive and have been mapped to chromosomes I (pbs2) and V (pbs1 and pbs3).