Power spectrum analysis of ultrasonic vocalization elicited by maternal separation in rat pups
Power spectrum analysis of ultrasonic vocalization elicited by maternal separation in rat pups
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DOI：
10.1016/j.brainres.2009.06.003
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发表时间：
2009-08-04
期刊：
影响因子：
2.9
通讯作者：
Ohta, Hisashi
中科院分区：
文献类型：
--
作者：
Ise, Satoko;Ohta, Hisashi
本研究探讨了环境刺激和药物如何改变母体分离诱导的超声发声的频率分布。使用10天的Sprague-Dawley大鼠幼鼠在各种环境温度下测量超声发声的数量和频率，并对促肾上腺皮质激素释放因子（CRF）和GABA能系统进行药理学操作。超声波发声由两个不同的峰值在30 kHz至50 kHz的频率范围内。当环境温度降低时，在高频范围内的曲线下面积（AUC）和超声发声的数量增加。选择性CRF 1受体拮抗剂NBI 27914和典型抗焦虑药地西泮的全身给药在24 ℃的环境温度下以剂量依赖性方式降低了高频范围内的AUC和超声发声次数。低频范围内的AUC不随环境温度的改变或用NBI 27914和地西泮处理而改变，除了高剂量（1 mg/kg）地西泮。这些结果表明，隔离幼鼠的情绪水平不仅反映了母亲分离引起的超声波发声的数量，而且还反映了频率分布。高频成分，但不是低频成分，超声发声的负性情感状态的变化是敏感的孤立的小狗。(C)2009 Elsevier B. V.保留所有权利。
The present study investigated how frequency distribution of maternal separation-induced ultrasonic vocalization was altered by environmental stimuli and pharmacological agents. Sprague-Dawley rat pups at 10 days were used to measure numbers and frequencies of the ultrasonic vocalizations under various ambient temperatures and with pharmacological manipulations of the corticotropin-releasing factor (CRF) and GABAergic systems. The ultrasonic vocalization consisted of two distinct peaks in the frequency range of 30 kHz to 50 kHz. The area under the curve (AUC) in the high-frequency range and the number of the ultrasonic vocalizations increased when ambient temperature was lowered. Systemic administration of a selective CRF1 receptor antagonist, NBI27914, and a typical anxiolytic, diazepam, decreased the AUC in the high-frequency range and the number of the ultrasonic vocalizations in a dose-dependent manner at an ambient temperature of 24 degrees C. The AUC in the low-frequency range did not change with an alteration in ambient temperature or treatment with NBI27914 and diazepam except a high dose (1 mg/kg) of diazepam. These results demonstrated that emotional levels of isolated pups reflected not only the number but also the frequency distribution of maternal separation-induced ultrasonic vocalizations. High-frequency components, but not low-frequency components, of the ultrasonic vocalization were sensitive to changes in negative affective state of isolated pups. (C) 2009 Elsevier B.V. All rights reserved.