Effect of NMDA on proliferation and apoptosis in hippocampal neural stem cells treated with MK-801
Effect of NMDA on proliferation and apoptosis in hippocampal neural stem cells treated with MK-801
复制标题
NMDA 对 MK-801 处理的海马神经干细胞增殖和凋亡的影响。
DOI：
10.3892/etm.2018.6346
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发表时间：
2018-08-01
影响因子：
2.7
通讯作者：
Liu, Juan
中科院分区：
文献类型：
--
作者：
Ding, Juan;Shao, Yu;Liu, Juan
本研究旨在探讨N-甲基-D-天冬氨酸（NMDA）对地佐环平（MK-801）处理的海马神经干细胞（NSCs）增殖和凋亡的影响。将培养的海马神经干细胞随机分为4组，分别为未处理对照组、MK-801处理组、NMDA处理组和MK-801 + NMDA处理组。通过MTS和流式细胞术测定每个实验组的凋亡和细胞凋亡应答。结果表明，MK-801和NMDA对海马神经干细胞的增殖有显著影响。与对照组相比，MK-801、NMDA和M + N处理组的细胞存活率降低。NMDA和M + N处理组的细胞存活率较MK-801处理组增加。MK-801和NMDA可显著影响海马神经干细胞的凋亡。与对照组相比，MK-801和NMDA组的总凋亡和早期凋亡均显著增加。与MK-801和NMDA组相比，M + N组神经干细胞凋亡总数和早期凋亡数均明显减少。MK-801和NMDA组神经干细胞晚期凋亡较对照组明显减少。与MK-801组和NMDA组相比，M + N组神经干细胞晚期凋亡明显增加。本研究表明，MK-801抑制海马神经干细胞的增殖和增加凋亡。NMDA可降低MK-801的神经毒性，这可能与其对NMDA受体的活性有关，可能是治疗精神分裂症的新靶点。
The purpose of the present study was to investigate effects of N-methyl-D-aspartate (NMDA) on proliferation and apoptosis of hippocampal neural stern cells (NSCs) treated with dizocilpine (MK-801). Cultures of hippocampal NSCs were randomly divided into four groups consisting of an untreated control, cells treated with MK-801, NMDA and a combination of MK801 and NMDA (M+N). Proliferative and apoptotic responses for each of the experimental groups were determined by MTS and flow cytometry. The results revealed that MK-801 and NMDA exerted significant effects on hippocampal NSCs proliferation. Cell survival rates decreased in MK-801, NMDA and M+N treated groups compared with the control group. Cells survival rates in NMDA and M+N treated groups increased compared with the MK-80l treated group. MK-801 and NMDA were demonstrated to significantly affect apoptosis in hippocampal NSCs. Total and early stages of apoptosis in MK-801 and NMDA groups significantly increased compared with the control group. Total and early apoptosis of NSCs in the M+N group significantly decreased compared with MK-801 and NMDA groups. Late apoptosis of NSCs in MK-801 and NMDA groups significantly decreased compared with the control group. Late apoptosis of NSCs in the M+N group significantly increased compared with MK-801 and NMDA groups. The present study revealed that MK-801 inhibited proliferation and increased apoptosis in hippocampal NSCs. NMDA may reduce the neurotoxicity induced by MK-801, which may be associated with its activity towards NMDA receptors and may describe a novel therapeutic target for the treatment of schizophrenia.