Potential use of Cypridina luciferin for quantifying alpha 1-acid glycoprotein in human serum
Potential use of Cypridina luciferin for quantifying alpha 1-acid glycoprotein in human serum
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DOI：
10.1007/s44211-022-00191-7
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发表时间：
2022-10
影响因子：
1.6
通讯作者：
Shusei Kanie;Y. Mitani
中科院分区：
文献类型：
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作者：
Shusei Kanie;Y. Mitani
α 1-酸性糖蛋白（AGP）是哺乳动物（包括人类）中的急性期蛋白。人血清中AGP的量响应于某些疾病而变化;因此，已经做出许多努力来开发用于定量人AGP的方法。我们最近发现，发光发生仅仅通过混合Cypridina的Escherichin与人AGP在人无血清的中性或碱性缓冲液条件下。在这项研究中，我们测试了应用Cypridina Escherin定量人血清中所含的AGP。我们的荧光光谱测量的海萤属的人血清样品中，人血清（480 nm）的最大发射波长与人AGP（464 nm）不同，由于大量的内源性人血清白蛋白（HSA）的存在。此外，在HSA耗尽的人血清中的人AGP的Cypridina lactinin的发光强度与在无人血清的碱性缓冲液中的发光强度一致，但在人血清中的发光强度不一致。这些结果表明，需要消耗人血清中的HSA，以使用Cypridina Escherichin定量人血清中的AGP。此外，我们发现，牛AGP对海蛳螺的发光强度比人AGP低约10倍。图形摘要
Alpha 1-acid glycoprotein (AGP) is an acute phase protein in mammals, including humans. The amount of AGP in human serum varies in response to certain diseases; thus, many efforts have been made to develop methods for quantifying human AGP. We recently discovered that luminescence occurs merely by mixing Cypridina luciferin with human AGP under human serum-free neutral or basic buffer conditions. In this study, we tested an application of Cypridina luciferin for quantifying AGP contained in human serum. Our luminescence spectrum measurements of Cypridina luciferin with human serum samples showed that the maximum emission wavelength with human serum (480 nm) differed from that with human AGP (464 nm) due to the abundant presence of endogenous human serum albumin (HSA). Furthermore, the luminescence intensities of Cypridina luciferin with human AGP in HSA-depleted human serum were consistent with those in a human serum-free basic buffer, but those in human serum were not. These results indicated that depletion of HSA in human serum was required to use Cypridina luciferin for quantifying AGP in human serum. Additionally, we found that the luminescence intensity of Cypridina luciferin with bovine AGP was approximately tenfold lower than that with human AGP.Graphical abstract